BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16321

Title: Backbone 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH8   PubMed: 19928833

Deposition date: 2009-05-28 Original release date: 2009-10-14

Authors: Serniwka, Stephanie; Shaw, Gary

Citation: Serniwka, Stephanie; Shaw, Gary. "The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site"  Biochemistry 48, 12169-12179 (2009).

Assembly members:
UbcH8, polymer, 152 residues, 17638 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UbcH8: MASMRVVKELEDLQKKPPPY LRNLSSDDANVLVWHALLLP DQPPYHLKAFNLRISFPPEY PFKPPMIKFTTKIYHPNVDE NGQICLPIISSENWKPSTKT SQVLEALNVLVNRPNIREPL RMDLADLLTQNPELFRKNAE EFTLRFGVDRPS

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts140
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH81

Entities:

Entity 1, UbcH8 152 residues - 17638 Da.

1   METALASERMETARGVALVALLYSGLULEU
2   GLUASPLEUGLNLYSLYSPROPROPROTYR
3   LEUARGASNLEUSERSERASPASPALAASN
4   VALLEUVALTRPHISALALEULEULEUPRO
5   ASPGLNPROPROTYRHISLEULYSALAPHE
6   ASNLEUARGILESERPHEPROPROGLUTYR
7   PROPHELYSPROPROMETILELYSPHETHR
8   THRLYSILETYRHISPROASNVALASPGLU
9   ASNGLYGLNILECYSLEUPROILEILESER
10   SERGLUASNTRPLYSPROSERTHRLYSTHR
11   SERGLNVALLEUGLUALALEUASNVALLEU
12   VALASNARGPROASNILEARGGLUPROLEU
13   ARGMETASPLEUALAASPLEULEUTHRGLN
14   ASNPROGLULEUPHEARGLYSASNALAGLU
15   GLUPHETHRLEUARGPHEGLYVALASPARG
16   PROSER

Samples:

sample_UbcH8: UbcH8, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; EDTA 1 mM; DTT 1 mM; sodium chloride 150 mM; Arginine 50 mM; Glutamic Acid 50 mM; H2O 90%; D2O 10%

sample_conditions_for_UbcH8: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_UbcH8isotropicsample_conditions_for_UbcH8
3D CBCA(CO)NHsample_UbcH8isotropicsample_conditions_for_UbcH8
3D HNCACBsample_UbcH8isotropicsample_conditions_for_UbcH8
3D HNCOsample_UbcH8isotropicsample_conditions_for_UbcH8
3D C(CO)NHsample_UbcH8isotropicsample_conditions_for_UbcH8
3D H(CCO)NHsample_UbcH8isotropicsample_conditions_for_UbcH8

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ v2.1B, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWS O14933
PDB
DBJ BAG38109
EMBL CAB64566 CAG33407
GB AAB86433 AAD17525 AAH32491 ABM82217 ABM85401
REF NP_004214 NP_937826 XP_003318029 XP_003826475 XP_004051215
SP O14933

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts