BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16328

Title: the S173N variant of rabbit prion protein (91-228)   PubMed: 20639199

Deposition date: 2009-05-30 Original release date: 2010-07-20

Authors: Li, Jun; Wen, Yi; Lin, Donghai

Citation: Wen, Yi; Li, Jun; Yao, Wenming; Xiong, Minqian; Hong, Jing; Peng, Yu; Xiao, Gengfu; Lin, Donghai. "Unique structural characteristics of the rabbit prion protein."  J. Biol. Chem. 285, 31682-31693 (2010).

Assembly members:
prion_protein, polymer, 145 residues, Formula weight is not available

Natural source:   Common Name: Rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
prion_protein: MQGGTHNQWGKPSKPKTSMK HVAGAAAAGAVVGGLGGYML GSAMSRPLIHFGNDYEDRYY RENMYRYPNQVYYRPVDQYS NQNNFVHDCVNITVKQHTVT TTTKGENFTETDIKIMERVV EQMCITQYQQESQAAYQRAH HHHHH

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts126
1H chemical shifts714

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1prion protein1

Entities:

Entity 1, prion protein 145 residues - Formula weight is not available

1   METGLNGLYGLYTHRHISASNGLNTRPGLY
2   LYSPROSERLYSPROLYSTHRSERMETLYS
3   HISVALALAGLYALAALAALAALAGLYALA
4   VALVALGLYGLYLEUGLYGLYTYRMETLEU
5   GLYSERALAMETSERARGPROLEUILEHIS
6   PHEGLYASNASPTYRGLUASPARGTYRTYR
7   ARGGLUASNMETTYRARGTYRPROASNGLN
8   VALTYRTYRARGPROVALASPGLNTYRSER
9   ASNGLNASNASNPHEVALHISASPCYSVAL
10   ASNILETHRVALLYSGLNHISTHRVALTHR
11   THRTHRTHRLYSGLYGLUASNPHETHRGLU
12   THRASPILELYSILEMETGLUARGVALVAL
13   GLUGLNMETCYSILETHRGLNTYRGLNGLN
14   GLUSERGLNALAALATYRGLNARGALAHIS
15   HISHISHISHISHIS

Samples:

sample: prion_protein, [U-100% 13C; U-100% 15N], 1 mM; sodium acetate 20 mM; H2O 90%; D2O 10%

sample_condition: ionic strength: 0.04 M; pH: 4.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
standard NMR experimentssampleisotropicsample_condition

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15394 15399 16616
PDB
EMBL CBK44083
GB AAC48697 AAD01554 ABL75505 AEM44421 AEM44422
REF NP_001075490 XP_008254357 XP_008254358
SP Q95211

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts