BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16363

Title: Solution structure of the Nuclear coactivator binding domain of CBP   PubMed: 20616042

Deposition date: 2009-06-24 Original release date: 2010-08-16

Authors: Kjaergaard, Magnus; Teilum, Kaare; Poulsen, Flemming

Citation: Kjaergaard, Magnus; Teilum, Kaare; Poulsen, Flemming. "Conformational selection in the molten globule state of the nuclear coactivator binding domain of CBP"  Proc. Natl. Acad. Sci. U S A. 107, 12535-12540 (2010).

Assembly members:
CBP, polymer, 59 residues, 6568.623 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CBP: PNRSISPSALQDLLRTLKSP SSPQQQQQVLNILKSNPQLM AAFIKQRTAKYVANQPGMQ

Data sets:
Data typeCount
13C chemical shifts236
15N chemical shifts49
1H chemical shifts372

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CBP1

Entities:

Entity 1, CBP 59 residues - 6568.623 Da.

1   PROASNARGSERILESERPROSERALALEU
2   GLNASPLEULEUARGTHRLEULYSSERPRO
3   SERSERPROGLNGLNGLNGLNGLNVALLEU
4   ASNILELEULYSSERASNPROGLNLEUMET
5   ALAALAPHEILELYSGLNARGTHRALALYS
6   TYRVALALAASNGLNPROGLYMETGLN

Samples:

sample_1: D2O 10%; sodium chloride 20 mM; sodium phosphate 20 mM; protein, [U-100% 13C; U-100% 15N], 2 mM; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 304 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

ARIA v2.2, Linge, O, . - structure solution

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15398 17073
PDB
DBJ BAE06125 BAI45616
GB AAB28651 AAC17736 AAC51331 AAC51340 AAC51770
PRF 1923401A
REF NP_001020603 NP_001073315 NP_001247644 NP_004371 NP_596872
SP P45481 Q6JHU9 Q92793

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts