BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16397

Title: Solution structure of SCA7 zinc finger domain from human ataxin-7 protein   PubMed: 20634802

Deposition date: 2009-07-08 Original release date: 2010-10-18

Authors: Wang, Ying-Hui; Atkinson, Andrew; Bonnet, Jacques; Romier, Christophe; Kieffer, Bruno

Citation: Bonnet, Jacques; Wang, Ying-Hui; Spedale, Gianpiero; Atkinson, R. Andrew; Romier, Christophe; Hamiche, Ali; Pijnappel, W. W M Pim; Timmers, H. Th Marc; Tora, Laszlo; Devys, Didier; Kieffer, Bruno. "The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties."  EMBO Rep. 11, 612-618 (2010).

Assembly members:
SCA7 domain, polymer, 74 residues, 6981.115 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SCA7 domain: GSKFLNKRLSEREFDPDIHC GVIDLDTKKPCTRSLTCKTH SLTQRRAVQGRRKRFDVLLA EHKNKTREKELIRH

Data sets:
Data typeCount
1H chemical shifts540

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SCA7 domain1
2ZINC ION2

Entities:

Entity 1, SCA7 domain 74 residues - 6981.115 Da.

1   GLYSERLYSPHELEUASNLYSARGLEUSER
2   GLUARGGLUPHEASPPROASPILEHISCYS
3   GLYVALILEASPLEUASPTHRLYSLYSPRO
4   CYSTHRARGSERLEUTHRCYSLYSTHRHIS
5   SERLEUTHRGLNARGARGALAVALGLNGLY
6   ARGARGLYSARGPHEASPVALLEULEUALA
7   GLUHISLYSASNLYSTHRARGGLULYSGLU
8   LEUILEARGHIS

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: sodium phosphate 50 mM; sodium chloride 200 mM; DTT 2 mM; entity_1, [U-99% 13C; U-99% 15N], 0.3 mM; H2O 90%; D2O 10%

sample_2: sodium phosphate 50 mM; sodium chloride 200 mM; DTT 2 mM; entity_1 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.3 M; pH: 7.1; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 950 MHz

Related Database Links:

PDB
DBJ BAC32834 BAG64969
EMBL CAA04154
GB AAC19163 AAC39765 AAI37847 AAI66666 AAL84238
REF NP_000324 NP_001121621 NP_001170858 NP_631973 XP_001174609
SP O15265 Q8R4I1
TPG DAA17075