BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16407

Title: Role of Trp-Trp interactions in Trpzip beta-hairpin formation, structure and stability   PubMed: 19788311

Deposition date: 2009-07-14 Original release date: 2009-08-25

Authors: Wu, Ling; McElheny, Dan; Huang, Rong; Keiderling, Timothy

Citation: Wu, Ling; McElheny, Dan; Huang, Rong; Keiderling, Timothy. "Role of tryptophan-tryptophan interactions in Trpzip beta-hairpin formation, structure, and stability."  Biochemistry 48, 10362-10371 (2009).

Assembly members:
W2W11, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
W2W11: SWTVENGKVTWK

Data sets:
Data typeCount
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 12 residues - Formula weight is not available

1   SERTRPTHRVALGLUASNGLYLYSVALTHR
2   TRPLYS

Samples:

sample_1: W2W11 4 ± 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 5.8; pressure: 1 atm; temperature: 281 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRView v5.2.2_01, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Guntert, Mumenthaler and Wuthrich, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking, refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz