BMRB Entry 16419
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16419
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Title: Local Perturbations by Ligand Binding of Hydrogen Deuterium Exchange Kinetics in a Four-helix Bundle Protein, Acyl Coenzyme A Binding Protein (ACBP) PubMed: 7623386
Deposition date: 2009-07-23 Original release date: 2012-06-06
Authors: Kragelund, Birthe; Knudsen, Jens; Poulsen, Flemming
Citation: Kragelund, B.; Knudsen, J.; Poulsen, F.. "Local perturbations by ligand binding of hydrogen deuterium exchange kinetics in a four-helix bundle protein, acyl coenzyme A binding protein (ACBP)." J. Mol. Biol. 250, 695-706 (1995).
Assembly members:
ACBP, polymer, 86 residues, 10043.79 Da.
TH3, non-polymer, 1003.927 Da.
Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
ACBP: SQAEFDKAAEEVKHLKTKPA
DEEMLFIYSHYKQATVGDIN
TERPGMLDFKGKAKWDAWNE
LKGTSKEDAMKAYIDKVEEL
KKKYGI
- assigned_chemical_shifts
- chemical_rates
| Data type | Count |
| chemical rates | 56 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ACBP | 1 |
| 2 | palmitoyl-CoA | 2 |
Entities:
Entity 1, ACBP 86 residues - 10043.79 Da.
| 1 | SER | GLN | ALA | GLU | PHE | ASP | LYS | ALA | ALA | GLU | ||||
| 2 | GLU | VAL | LYS | HIS | LEU | LYS | THR | LYS | PRO | ALA | ||||
| 3 | ASP | GLU | GLU | MET | LEU | PHE | ILE | TYR | SER | HIS | ||||
| 4 | TYR | LYS | GLN | ALA | THR | VAL | GLY | ASP | ILE | ASN | ||||
| 5 | THR | GLU | ARG | PRO | GLY | MET | LEU | ASP | PHE | LYS | ||||
| 6 | GLY | LYS | ALA | LYS | TRP | ASP | ALA | TRP | ASN | GLU | ||||
| 7 | LEU | LYS | GLY | THR | SER | LYS | GLU | ASP | ALA | MET | ||||
| 8 | LYS | ALA | TYR | ILE | ASP | LYS | VAL | GLU | GLU | LEU | ||||
| 9 | LYS | LYS | LYS | TYR | GLY | ILE |
Entity 2, palmitoyl-CoA - C37 H64 N7 O17 P3 S - 1003.927 Da.
| 1 | TH3 |
Samples:
sample_1: ACBP 2 mM; potassium phosphate 0.04 mM; palmitoyl-CoA 2 mM; D2O 100%
sample_conditions_1: pH: 6.65; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
PRONTO, Kjur, Andersen and Poulsen - data analysis, processing
MNMR - processing
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 2049 2050 |
| PDB | |
| EMBL | CAA44618 |
| GB | AAA30495 AAI14182 AIM41257 ELR45212 |
| REF | NP_001106792 XP_004004799 XP_005910864 XP_006040065 XP_006057227 |
| SP | P07107 |
| TPG | DAA32604 |