BMRB Entry 16425

Name: Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex
Published: 2010-07-27

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PDB ID: 2km8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16425
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex PubMed: 20600122

Deposition date: 2009-07-24 Original release date: 2010-07-27

Authors: Leeper, Thomas; Varani, Gabriele

Citation: Leeper, Thomas; Qu, Xiangping; Lu, Connie; Moore, Claire; Varani, Gabriele. "Novel Protein-Protein Contacts Facilitate mRNA 3'-Processing Signal Recognition by Rna15 and Hrp1." J. Mol. Biol. 401, 334-349 (2010).

Assembly members:
rna15p, polymer, 84 residues, Formula weight is not available
anchor_RNA, polymer, 13 residues, 112.088 Da.
hrp1p, polymer, 167 residues, Formula weight is not available

Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
rna15p: NPPSRVVYLGSIPYDQTEEQ ILDLCSNVGPVINLKMMFDP QTGRSKGYAFIEFRDLESSA SAVRNLNGYQLGSRFLKCGY SSNS
anchor_RNA: UAUAUAUAAUAAU
hrp1p: KESCKMFIGGLNWDTTEDNL REYFGKYGTVTDLKIMKDPA TGRSRGFGFLSFEKPSSVDE VVKTQHILDGKVIDPKRAIP RDEQDKTGKIFVGGIGPDVR PKEFEEFFSQWGTIIDAQLM LDKDTGQSRGFGFVTYDSAD AVDRVCQNKFIDFKDRKIEI KRAEPRH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	88
1H chemical shifts	554

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	anisotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	anisotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	anisotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	anisotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
3D HNCO	sample_5	isotropic	sample_conditions_1
3D HNCA	sample_5	isotropic	sample_conditions_1
3D HNCACB	sample_5	isotropic	sample_conditions_1
3D HN(CO)CA	sample_5	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_5	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_5	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_5	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_6	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_6	isotropic	sample_conditions_1

PDB	2KM8 2X1A 2X1B 2X1F 2CJK 2KM8
DBJ	GAA23344 GAA26207
EMBL	CAA96746 CAY79715 CAA64546 CAA99142 CAY86168
GB	AAA34984 AHY79328 AJP38746 AJR76066 AJR76567 AAA79097 AAB18142 AHY77195 EDN63753 EDV10496
REF	NP_011471 NP_014518 XP_011105369
SP	P25299 Q99383
TPG	DAA08056 DAA10661

Biological Magnetic Resonance Data Bank

BMRB Entry 16425

Title: Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex PubMed: 20600122

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

Entity Assembly ID	Entity Name	Entity ID
1	rna15p	1
2	anchor_RNA	2
3	hrp1p	3