BMRB Entry 16456

Assembly:

Entities:

Entity 1, TRAP-TSR 49 residues - Formula weight is not available

Entity 2, heparin 225 residues - 24745.02 Da.

Samples:

sample_1: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; D2O 6%; H2O 94%

sample_2: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; heparin 0.1 mM; D2O 6%; H2O 94%

sample_3: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; heparin 0.2 mM; D2O 6%; H2O 94%

sample_4: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; heparin 0.3 mM; D2O 6%; H2O 94%

sample_5: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; heparin 0.4 mM; D2O 6%; H2O 94%

sample_6: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; heparin 0.6 mM; D2O 6%; H2O 94%

sample_7: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; heparin 1.25 mM; D2O 6%; H2O 94%

sample_8: TRAP-TSR, [U-13C; U-15N], 0.5 mM; bis-Tris buffer 20 mM; heparin 1.9 mM; D2O 6%; H2O 94%

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 283 K

Experiments:

Software:

SPARKY, Goddard - processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER v8, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - energy minimization

ProcheckNMR, Laskowski and MacArthur - data analysis

Molmol, Koradi, Billeter and Wuthrich - visualization

NMR spectrometers:

• Varian Unity Inova 500 MHz
• Varian Unity Inova 600 MHz