BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16471

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for IQGAP1 (401-533)   PubMed: 20075861

Deposition date: 2009-08-27 Original release date: 2010-01-28

Authors: Smith, Matthew; Ikura, Mitsu

Citation: Smith, Matthew; Hardy, W. Rod; Li, Guang-Yao; Goudreault, Marilyn; Hersch, Steven; Metalnikov, Pavel; Starostine, Andrei; Pawson, Tony; Ikura, Mitsuhiko. "The PTB domain of ShcA couples receptor activation to the cytoskeletal regulator IQGAP1."  EMBO J. 29, 884-896 (2010).

Assembly members:
IQGAP1_(401-533), polymer, 133 residues, 14888 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IQGAP1_(401-533): KGIAEKTVLELMNPEAQLPQ VYPFAADLYQKELATLQQQS PEHSLTHPELTVAVEMLSSV ALINRALESGDMTTVWKQLS SSVTGLTNIEEENCQRYLDE LMKLKAQAHAENNAFITWND IQACVDHVNLVVH

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts115
1H chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ShcA PTB Domain Ligand1

Entities:

Entity 1, ShcA PTB Domain Ligand 133 residues - 14888 Da.

1   LYSGLYILEALAGLULYSTHRVALLEUGLU
2   LEUMETASNPROGLUALAGLNLEUPROGLN
3   VALTYRPROPHEALAALAASPLEUTYRGLN
4   LYSGLULEUALATHRLEUGLNGLNGLNSER
5   PROGLUHISSERLEUTHRHISPROGLULEU
6   THRVALALAVALGLUMETLEUSERSERVAL
7   ALALEUILEASNARGALALEUGLUSERGLY
8   ASPMETTHRTHRVALTRPLYSGLNLEUSER
9   SERSERVALTHRGLYLEUTHRASNILEGLU
10   GLUGLUASNCYSGLNARGTYRLEUASPGLU
11   LEUMETLYSLEULYSALAGLNALAHISALA
12   GLUASNASNALAPHEILETHRTRPASNASP
13   ILEGLNALACYSVALASPHISVALASNLEU
14   VALVALHIS

Samples:

sample_1: IQGAP1 (401-533), [U-13C; U-15N], 200 uM; D2O 10%; H2O 90%; NaCl 100 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

SWS Q9JKF1
DBJ BAC97854 BAE37174 BAE37805
GB AAF60344 AAH22105 AAH37685 AAH46385 EDL06977
REF NP_001101959 NP_057930 XP_003513458 XP_005077870 XP_005357708
SP Q9JKF1

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts