BMRB Entry 16471
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16471
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for IQGAP1 (401-533) PubMed: 20075861
Deposition date: 2009-08-27 Original release date: 2010-01-28
Authors: Smith, Matthew; Ikura, Mitsu
Citation: Smith, Matthew; Hardy, W. Rod; Li, Guang-Yao; Goudreault, Marilyn; Hersch, Steven; Metalnikov, Pavel; Starostine, Andrei; Pawson, Tony; Ikura, Mitsuhiko. "The PTB domain of ShcA couples receptor activation to the cytoskeletal regulator IQGAP1." EMBO J. 29, 884-896 (2010).
Assembly members:
IQGAP1_(401-533), polymer, 133 residues, 14888 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
IQGAP1_(401-533): KGIAEKTVLELMNPEAQLPQ
VYPFAADLYQKELATLQQQS
PEHSLTHPELTVAVEMLSSV
ALINRALESGDMTTVWKQLS
SSVTGLTNIEEENCQRYLDE
LMKLKAQAHAENNAFITWND
IQACVDHVNLVVH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 237 |
15N chemical shifts | 115 |
1H chemical shifts | 115 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ShcA PTB Domain Ligand | 1 |
Entities:
Entity 1, ShcA PTB Domain Ligand 133 residues - 14888 Da.
1 | LYS | GLY | ILE | ALA | GLU | LYS | THR | VAL | LEU | GLU | ||||
2 | LEU | MET | ASN | PRO | GLU | ALA | GLN | LEU | PRO | GLN | ||||
3 | VAL | TYR | PRO | PHE | ALA | ALA | ASP | LEU | TYR | GLN | ||||
4 | LYS | GLU | LEU | ALA | THR | LEU | GLN | GLN | GLN | SER | ||||
5 | PRO | GLU | HIS | SER | LEU | THR | HIS | PRO | GLU | LEU | ||||
6 | THR | VAL | ALA | VAL | GLU | MET | LEU | SER | SER | VAL | ||||
7 | ALA | LEU | ILE | ASN | ARG | ALA | LEU | GLU | SER | GLY | ||||
8 | ASP | MET | THR | THR | VAL | TRP | LYS | GLN | LEU | SER | ||||
9 | SER | SER | VAL | THR | GLY | LEU | THR | ASN | ILE | GLU | ||||
10 | GLU | GLU | ASN | CYS | GLN | ARG | TYR | LEU | ASP | GLU | ||||
11 | LEU | MET | LYS | LEU | LYS | ALA | GLN | ALA | HIS | ALA | ||||
12 | GLU | ASN | ASN | ALA | PHE | ILE | THR | TRP | ASN | ASP | ||||
13 | ILE | GLN | ALA | CYS | VAL | ASP | HIS | VAL | ASN | LEU | ||||
14 | VAL | VAL | HIS |
Samples:
sample_1: IQGAP1 (401-533), [U-13C; U-15N], 200 uM; D2O 10%; H2O 90%; NaCl 100 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
SWS | Q9JKF1 |
DBJ | BAC97854 BAE37174 BAE37805 |
GB | AAF60344 AAH22105 AAH37685 AAH46385 EDL06977 |
REF | NP_001101959 NP_057930 XP_003513458 XP_005077870 XP_005357708 |
SP | Q9JKF1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts