BMRB Entry 16481
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16481
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Title: 1H, 13C and 15N resonance assignments of the hyperthermostable 264-residue endo-<beta>-1,3-glucanase LamA at 62 C PubMed: 19913513
Deposition date: 2009-09-07 Original release date: 2009-11-20
Authors: Ippel, Johannes; Koutsopoulos, Sotirios; Nabuurs, Sanne; van Berkel, Willem; van der Oost, John; van Mierlo, Carlo
Citation: Ippel, Johannes; Koutsopoulos, Sotirios; Nabuurs, Sanne; van Berkel, Willem; van der Oost, John; van Mierlo, Carlo. "NMR characterization of a 264-residue hyperthermostable endo-beta-1,3-glucanase." Biochem. Biophys. Res. Commun. 391, 370-375 (2010).
Assembly members:
pfLamA, polymer, 264 residues, 30085 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
pfLamA: MVPEVIEIDGKQWRLIWHDE
FEGSEVNKEYWTFEKGNGIA
YGIPGWGNGELEYYTENNTY
IVNGTLVIEARKEIITDPNE
GTFLYTSSRLKTEGKVEFSP
PVVVEARIKLPKGKGLWPAF
WMLGSNIREVGWPNCGEIDI
MEFLGHEPRTIHGTVHGPGY
SGSKGITRAYTLPEGVPDFT
EDFHVFGIVWYPDKIKWYVD
GTFYHEVTKEQVEAMGYEWV
FDKPFYIILNLAVGGYWPGN
PDATTPFPAKMVVDYVRVYS
FVSG
| Data type | Count |
| 13C chemical shifts | 1799 |
| 15N chemical shifts | 526 |
| 1H chemical shifts | 1961 |