BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16514

Title: NMR chemical shifts of soluble synaptobrevin (1-96) in buffer and in DPC micelle   PubMed: 19918058

Deposition date: 2009-09-23 Original release date: 2009-11-20

Authors: Ellena, Jeffrey; Liang, Binyong; Wiktor, Maciej; Stein, Alexander; Cafiso, David; Jahn, Reinhard; Tamm, Lukas

Citation: Ellena, Jeffrey; Liang, Binyong; Wiktor, Maciej; Stein, Alexander; Cafiso, David; Jahn, Reinhard; Tamm, Lukas. "Dynamic structure of lipid-bound synaptobrevin suggests a nucleation-propagation mechanism for trans-SNARE complex formation."  Proc. Natl. Acad. Sci. U.S.A. 106, 20306-20311 (2009).

Assembly members:
syb96, polymer, 96 residues, Formula weight is not available

Natural source:   Common Name: Norway Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
syb96: MSATAATVPPAAPAGEGGPP APPPNLTSNRRLQQTQAQVD EVVDIMRVNVDKVLERDQKL SELDDRADALQAGASQFETS AAKLKRKYWWKNLKMM

Data sets:
Data typeCount
13C chemical shifts543
15N chemical shifts174
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1syb961

Entities:

Entity 1, syb96 96 residues - Formula weight is not available

1   METSERALATHRALAALATHRVALPROPRO
2   ALAALAPROALAGLYGLUGLYGLYPROPRO
3   ALAPROPROPROASNLEUTHRSERASNARG
4   ARGLEUGLNGLNTHRGLNALAGLNVALASP
5   GLUVALVALASPILEMETARGVALASNVAL
6   ASPLYSVALLEUGLUARGASPGLNLYSLEU
7   SERGLULEUASPASPARGALAASPALALEU
8   GLNALAGLYALASERGLNPHEGLUTHRSER
9   ALAALALYSLEULYSARGLYSTYRTRPTRP
10   LYSASNLEULYSMETMET

Samples:

sample_1: syb96, [U-99% 13C; U-99% 15N], 0.2 mM; potassium phosphate 60 mM; NaCl 300 mM; DTT 5 mM; EDTA 1 mM; H2O 95%; D2O 5%

sample_2: syb96, [U-99% 13C; U-99% 15N], 0.5 mM; DPC 300 mM; potassium phosphate 60 mM; NaCl 300 mM; DTT 5 mM; EDTA 1 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 300 mM; pH: 6.1; pressure: 1 atm; temperature: 291 K

sample_conditions_2: ionic strength: 300 mM; pH: 6.1; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HNCAsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D HN(CA)COsample_2isotropicsample_conditions_2

Software:

SPARKY, Goddard - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16508 4272
PDB
DBJ BAC41125 BAE90084 BAF82244 BAG73857
EMBL CAA12385 CAA53792 CAB43509 CDQ77958
GB AAA42321 AAA60604 AAB03463 AAB62931 AAB88138
REF NP_001027992 NP_001034578 NP_001080944 NP_001088233 NP_001272142
SP P47193 P63026 P63027 P63044 P63045
TPG DAA18807

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts