BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16515

Title: Solution structure of mouse Par-3 PDZ2 (residues 450-558)

Deposition date: 2009-09-23 Original release date: 2010-05-13

Authors: Volkman, B.; Tyler, R.; Peterson, F.

Citation: Markley, J.; Bahrami, A.; Eghbalnia, H.; Peterson, F.; Ulrich, E.; Westler, W.; Volkman, B.. "Macromolecular Structure Determination by NMR Sepectroscopy"  (2009).

Assembly members:
mPar3_PDZ2, polymer, 111 residues, 11927.798 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mPar3_PDZ2: GSVYNTKKVGKRLNIQLKKG TEGLGFSITSRDVTIGGSAP IYVKNILPRGAAIQDGRLKA GDRLIEVNGVDLAGKSQEEV VSLLRSTKMEGTVSLLVFRQ EEAFHPREMNA

Data sets:
Data typeCount
13C chemical shifts407
15N chemical shifts108
1H chemical shifts621

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mPar3 PDZ21

Entities:

Entity 1, mPar3 PDZ2 111 residues - 11927.798 Da.

1   GLYSERVALTYRASNTHRLYSLYSVALGLY
2   LYSARGLEUASNILEGLNLEULYSLYSGLY
3   THRGLUGLYLEUGLYPHESERILETHRSER
4   ARGASPVALTHRILEGLYGLYSERALAPRO
5   ILETYRVALLYSASNILELEUPROARGGLY
6   ALAALAILEGLNASPGLYARGLEULYSALA
7   GLYASPARGLEUILEGLUVALASNGLYVAL
8   ASPLEUALAGLYLYSSERGLNGLUGLUVAL
9   VALSERLEULEUARGSERTHRLYSMETGLU
10   GLYTHRVALSERLEULEUVALPHEARGGLN
11   GLUGLUALAPHEHISPROARGGLUMETASN
12   ALA

Samples:

sample: mPar3 PDZ2, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 54 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sampleisotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

SPSCAN v1.1.0, R.W. Glaser - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

NMR spectrometers:

  • Bruker Avance II 600 MHz

Related Database Links:

BMRB 16520
PDB
REF XP_011836158

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts