BMRB Entry 16567
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16567
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Title: 1H, 13C, and 15N chemical shift assignments for the RNA recognition motif of Nab3 PubMed: 20309651
Deposition date: 2009-10-19 Original release date: 2010-05-04
Authors: Stefl, Richard; Kubicek, Karel; Pergoli, Roberto; Hobor, Fruzsina; Pasulka, Josef
Citation: Pergoli, Roberto; Kubicek, Karel; Hobor, Fruzsina; Pasulka, Josef; Stefl, Richard. "1H, 13C, and 15N chemical shift assignments for the RNA recognition motif of Nab3." Biomol. NMR Assignments 4, 119-121 (2010).
Assembly members:
RNA_binding_polypeptide, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RNA_binding_polypeptide: MTEMHNIPPKSRLFIGNLPL
KNVSKEDLFRIFSPYGHIMQ
INIKNAFGFIQFDNPQSVRD
AIECESQEMNFGKKLILEVS
SSNARPQFDHGDHGTNLEHH
HHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 201 |
15N chemical shifts | 60 |
1H chemical shifts | 376 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 104 residues - Formula weight is not available
1 | MET | THR | GLU | MET | HIS | ASN | ILE | PRO | PRO | LYS | ||||
2 | SER | ARG | LEU | PHE | ILE | GLY | ASN | LEU | PRO | LEU | ||||
3 | LYS | ASN | VAL | SER | LYS | GLU | ASP | LEU | PHE | ARG | ||||
4 | ILE | PHE | SER | PRO | TYR | GLY | HIS | ILE | MET | GLN | ||||
5 | ILE | ASN | ILE | LYS | ASN | ALA | PHE | GLY | PHE | ILE | ||||
6 | GLN | PHE | ASP | ASN | PRO | GLN | SER | VAL | ARG | ASP | ||||
7 | ALA | ILE | GLU | CYS | GLU | SER | GLN | GLU | MET | ASN | ||||
8 | PHE | GLY | LYS | LYS | LEU | ILE | LEU | GLU | VAL | SER | ||||
9 | SER | SER | ASN | ALA | ARG | PRO | GLN | PHE | ASP | HIS | ||||
10 | GLY | ASP | HIS | GLY | THR | ASN | LEU | GLU | HIS | HIS | ||||
11 | HIS | HIS | HIS | HIS |
Samples:
sample_1: RNA binding polypeptide, [U-99% 13C; U-99% 15N], 3 mM
sample_conditions_1: ionic strength: 0.3 M; pH: 8; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin, Goddard - chemical shift assignment, collection
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 900 MHz
Related Database Links:
PDB | |
DBJ | BAA07154 GAA26786 |
EMBL | CAA97903 CAY86769 |
GB | AAA81910 AHY77991 AJP41959 AJU23403 AJU24091 |
PRF | 2201484A |
REF | NP_015134 |
SP | P38996 |
TPG | DAA11244 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts