BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16633

Title: Solution structure of Bv8   PubMed: 20677202

Deposition date: 2009-12-14 Original release date: 2010-08-12

Authors: Daly, Norelle; Craik, David

Citation: Morales, Rodrigo; Daly, Norelle; Vetter, Irina; Mobli, Mehdi; Napier, Ian; Craik, David; Lewis, Richard; Christie, Macdonald; King, Glenn; Alewood, Paul; Durek, Thomas. "Chemical synthesis and structure of the prokineticin bv8."  Chembiochem 11, 1882-1888 (2010).

Assembly members:
prokineticin_Bv8, polymer, 77 residues, 8042.250 Da.

Natural source:   Common Name: yellow-bellied toad   Taxonomy ID: 8348   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bombina variegata

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
prokineticin_Bv8: AVITGACDKDVQCGSGTCCA ASAWSRNIRFCIPLGNSGED CHPASHKVPYDGKRLSSLCP CKSGLTCSKSGEKFKCS

Data sets:
Data typeCount
1H chemical shifts443

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1prokineticin_Bv81

Entities:

Entity 1, prokineticin_Bv8 77 residues - 8042.250 Da.

1   ALAVALILETHRGLYALACYSASPLYSASP
2   VALGLNCYSGLYSERGLYTHRCYSCYSALA
3   ALASERALATRPSERARGASNILEARGPHE
4   CYSILEPROLEUGLYASNSERGLYGLUASP
5   CYSHISPROALASERHISLYSVALPROTYR
6   ASPGLYLYSARGLEUSERSERLEUCYSPRO
7   CYSLYSSERGLYLEUTHRCYSSERLYSSER
8   GLYGLULYSPHELYSCYSSER

Samples:

sample_1: entity 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - collection

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
GB AAD45816
SP Q9PW66