BMRB Entry 16679
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16679
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C and 15N assignment of the C terminal domain of GNA2132 from Neisseria meningitidis PubMed: 20300890
Deposition date: 2010-01-14 Original release date: 2010-04-08
Authors: Esposito, Veronica; Veggi, Daniele; Musi, Valeria; Pizza, Mariagrazia; Pastore, Annalisa
Citation: Esposito, Veronica; Musi, Valeria; Veggi, Daniele; Pastore, Annalisa; Pizza, Mariagrazia. "1H, 13C and 15N assignment of the C-terminal domain of GNA2132 from Neisseria meningitidis." Biomol. NMR Assignments 4, 107-109 (2010).
Assembly members:
GNA_2132, polymer, 193 residues, 20609.7 Da.
Natural source: Common Name: Neisseria meningitidis Taxonomy ID: 487 Superkingdom: Eubacteria Kingdom: not available Genus/species: Neisseria meningitidis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
GNA_2132: MASLPAEMPLIPVNQADTLI
VDGEAVSLTGHSGNIFAPEG
NYRYLTYGAEKLPGGSYALR
VQGEPAKGEMLAGTAVYNGE
VLHFHTENGRPYPTRGRFAA
KVDFGSKSVDGIIDSGDDLH
MGTQKFKAAIDGNGFKGTWT
ENGGGDVSGRFYGPAGEEVA
GKYSYRPTDAEKGGFGVFAG
KKEQDLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 524 |
| 15N chemical shifts | 133 |
| 1H chemical shifts | 820 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GNA 2132 | 1 |
Entities:
Entity 1, GNA 2132 193 residues - 20609.7 Da.
| 1 | MET | ALA | SER | LEU | PRO | ALA | GLU | MET | PRO | LEU | ||||
| 2 | ILE | PRO | VAL | ASN | GLN | ALA | ASP | THR | LEU | ILE | ||||
| 3 | VAL | ASP | GLY | GLU | ALA | VAL | SER | LEU | THR | GLY | ||||
| 4 | HIS | SER | GLY | ASN | ILE | PHE | ALA | PRO | GLU | GLY | ||||
| 5 | ASN | TYR | ARG | TYR | LEU | THR | TYR | GLY | ALA | GLU | ||||
| 6 | LYS | LEU | PRO | GLY | GLY | SER | TYR | ALA | LEU | ARG | ||||
| 7 | VAL | GLN | GLY | GLU | PRO | ALA | LYS | GLY | GLU | MET | ||||
| 8 | LEU | ALA | GLY | THR | ALA | VAL | TYR | ASN | GLY | GLU | ||||
| 9 | VAL | LEU | HIS | PHE | HIS | THR | GLU | ASN | GLY | ARG | ||||
| 10 | PRO | TYR | PRO | THR | ARG | GLY | ARG | PHE | ALA | ALA | ||||
| 11 | LYS | VAL | ASP | PHE | GLY | SER | LYS | SER | VAL | ASP | ||||
| 12 | GLY | ILE | ILE | ASP | SER | GLY | ASP | ASP | LEU | HIS | ||||
| 13 | MET | GLY | THR | GLN | LYS | PHE | LYS | ALA | ALA | ILE | ||||
| 14 | ASP | GLY | ASN | GLY | PHE | LYS | GLY | THR | TRP | THR | ||||
| 15 | GLU | ASN | GLY | GLY | GLY | ASP | VAL | SER | GLY | ARG | ||||
| 16 | PHE | TYR | GLY | PRO | ALA | GLY | GLU | GLU | VAL | ALA | ||||
| 17 | GLY | LYS | TYR | SER | TYR | ARG | PRO | THR | ASP | ALA | ||||
| 18 | GLU | LYS | GLY | GLY | PHE | GLY | VAL | PHE | ALA | GLY | ||||
| 19 | LYS | LYS | GLU | GLN | ASP | LEU | GLU | HIS | HIS | HIS | ||||
| 20 | HIS | HIS | HIS |
Samples:
sample: GNA 2132, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS 20 mM; sodium chloride 50 mM; DPC 30 mM
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample | isotropic | sample_conditions_1 |
| 3D HNCA | sample | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample | isotropic | sample_conditions_1 |
| 3D HNCO | sample | isotropic | sample_conditions_1 |
| 3D HNCACB | sample | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample | isotropic | sample_conditions_1 |
Software:
SPARKY v3.114, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| EMBL | CAX51093 CBA09711 CBN88224 CBX21586 CCA45797 |
| GB | AAF42440 AAF42567 AAF42570 AAF42571 AAF42572 |
| REF | NP_275117 WP_002218235 WP_002223217 WP_002224198 WP_002224716 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts