BMRB Entry 16746

Assembly:

Entities:

Entity 1, GmR58A 122 residues - 13133.925 Da.

LEHHHHHH are at the C-terminal as part of Hig-Tag

Samples:

NC_Sample: GmR58A, [U-100% 13C; U-100% 15N], 0.77 mM; sodium azide 2%; DTT 10 mM; CaCl 5 mM; sodium chloride 200 mM; MES 20 mM; H2O 95%; D2O 5%

PEG: GmR58A, [U-100% 13C; U-100% 15N], 0.65 mM; sodium azide 2%; DTT 10 mM; CaCl 5 mM; sodium chloride 200 mM; MES 20 mM; C12E5 4.2%; H2O 95%; D2O 5%

Negative_Gel: GmR58A, [U-100% 13C; U-100% 15N], 0.65 mM; sodium azide 2%; DTT 10 mM; CaCl 5 mM; sodium chloride 200 mM; MES 20 mM; negatively charged compressed polyacrylamide gel 7%; H2O 95%; D2O 5%

Stretch_Gel: GmR58A, [U-100% 13C; U-100% 15N], 0.65 mM; sodium azide 2%; DTT 10 mM; CaCl 5 mM; sodium chloride 200 mM; MES 20 mM; Neutral stretched polyacrylamide gel 5%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement

SPARKY v3.115, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PSVS, Bhattacharya and Montelione - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

• Varian INOVA 600 MHz