BMRB Entry 16759
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16759
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Title: Mapping of the anion binding sites on cytochrome c by differential chemical modification of lysine residues PubMed: 6254024
Deposition date: 2010-03-03 Original release date: 2010-03-24
Authors: Osheroff, Neil; Brautigan, David; Margoliash, E.
Citation: Osheroff, Neil; Brautigan, David; Margoliash, E.. "Mapping of anion binding sites on cytochrome c by differential chemical modification of lysine residues" Proc. Natl. Acad. Sci. U.S.A. 77, 4439-4443 (1980).
Assembly members:
cytochrome_c, polymer, 104 residues, Formula weight is not available
PO4, non-polymer, 94.971 Da.
Natural source: Common Name: Horse Taxonomy ID: 9796 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Equus caballus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
cytochrome_c: GDVEKGKKIFVQKCAQCHTV
EKGGKHKTGPNLHGLFGRKT
GQAPGFTYTDANKNKGITWK
EETLMEYLENPKKYIPGTKM
IFAGIKKKTEREDLIAYLKK
ATNE
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | cytochrome c | 1 |
2 | PO4 | 2 |
Entities:
Entity 1, cytochrome c 104 residues - Formula weight is not available
1 | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | PHE | ||||
2 | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | VAL | ||||
3 | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | PRO | ||||
4 | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | THR | ||||
5 | GLY | GLN | ALA | PRO | GLY | PHE | THR | TYR | THR | ASP | ||||
6 | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | LYS | ||||
7 | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ASN | ||||
8 | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | ||||
9 | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | GLU | ||||
10 | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | LYS | ||||
11 | ALA | THR | ASN | GLU |
Entity 2, PO4 - O4 P - 94.971 Da.
1 | PO4 |
Samples:
sample_1: PO4 8 mM; cytochrome c 10 mM; citrate2 32 mM; EDTA 0.2 mM; H2O 90%; D2O 10%
sample_2: PO4 8 mM; cytochrome c 10 mM; Cl2 32 mM; EDTA 0.2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
31P NMR | sample_1 | isotropic | sample_conditions_1 |
31P NMR | sample_2 | isotropic | sample_conditions_1 |
Software:
not_given, not given - not given
NMR spectrometers:
- JEOL FX-90Q 90 MHz
Related Database Links:
BMRB | 1058 1107 1108 1109 1110 1111 1112 1113 1114 1116 1170 1171 1404 17120 17340 1736 1783 17847 17848 1785 1787 1789 216 220 224 2366 2367 2368 243 244 25640 274 285 286 316 317 336 4189 436 437 438 439 |
PDB | |
GB | AAB33495 AAI05398 AAX77008 ABA06541 AEB61027 |
PRF | 610169A 711086A |
REF | NP_001039526 NP_001123442 NP_001157486 XP_004007999 XP_004418964 |
SP | P00004 P62894 P62895 P62896 P68096 |
TPG | DAA30512 |