BMRB Entry 16767

Name: Human Regenerating Gene Type IV (REG IV) PROTEIN, P91S mutant
Published: 2010-08-12

Click here to enlarge.

PDB ID: 2kv3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16767
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Human Regenerating Gene Type IV (REG IV) PROTEIN, P91S mutant PubMed: 20692269

Deposition date: 2010-03-04 Original release date: 2010-08-12

Authors: Ho, Meng-Ru; Lou, Yuan-Chao; Chen, Chinpan

Citation: Ho, Meng-Ru; Lou, Yuan-Chao; Wei, Shu-Yi; Luo, Shih-Chi; Lin, Wen-Chang; Lyu, Ping-Chiang; Chen, Chinpan. "Human RegIV Protein Adopts a Typical C-Type Lectin Fold but Binds Mannan with Two Calcium-Independent Sites." J. Mol. Biol. 402, 682-695 (2010).

Assembly members:
P91S, polymer, 130 residues, 16301 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
P91S: QSCAPGWFYHKSNCYGYFRK LRNWSDAELECQSYGNGAHL ASILSLKEASTIAEYISGYQ RSQSIWIGLHDPQKRQQWQW IDGAMYLYRSWSGKSMGGNK HCAEMSSNNNFLTWSSNECN KRQHFLCKYR

Data sets:

assigned_chemical_shifts
- assignments_chemical_shifts

Data type	Count
13C chemical shifts	523
15N chemical shifts	145
1H chemical shifts	805

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	P91S	1

Entities:

Entity 1, P91S 130 residues - 16301 Da.

1	GLN	SER	CYS	ALA	PRO	GLY	TRP	PHE	TYR	HIS
2	LYS	SER	ASN	CYS	TYR	GLY	TYR	PHE	ARG	LYS
3	LEU	ARG	ASN	TRP	SER	ASP	ALA	GLU	LEU	GLU
4	CYS	GLN	SER	TYR	GLY	ASN	GLY	ALA	HIS	LEU
5	ALA	SER	ILE	LEU	SER	LEU	LYS	GLU	ALA	SER
6	THR	ILE	ALA	GLU	TYR	ILE	SER	GLY	TYR	GLN
7	ARG	SER	GLN	SER	ILE	TRP	ILE	GLY	LEU	HIS
8	ASP	PRO	GLN	LYS	ARG	GLN	GLN	TRP	GLN	TRP
9	ILE	ASP	GLY	ALA	MET	TYR	LEU	TYR	ARG	SER
10	TRP	SER	GLY	LYS	SER	MET	GLY	GLY	ASN	LYS
11	HIS	CYS	ALA	GLU	MET	SER	SER	ASN	ASN	ASN
12	PHE	LEU	THR	TRP	SER	SER	ASN	GLU	CYS	ASN
13	LYS	ARG	GLN	HIS	PHE	LEU	CYS	LYS	TYR	ARG

Samples:

sample_1: Human Regenerating Gene Type IV, [U-99% 15N], 1 mM; Human Regenerating Gene Type IV, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 4.5; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	2KV3
GB	AAG02562 AAH17089 AAK48435 AAK59869 AAM95598
REF	NP_001152824 NP_114433 XP_001139975 XP_003268113 XP_003268114
SP	Q9BYZ8

Biological Magnetic Resonance Data Bank