BMRB Entry 16814
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16814
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Title: Structure of the three-Cys2His2 domain of mouse testis zinc finger protein PubMed: 20544958
Deposition date: 2010-03-31 Original release date: 2010-07-27
Authors: Chou, Chun-Chi; Lou, Yuan-Chao; Chen, Chinpan
Citation: Chou, Chun-Chi; Lou, Yuan-Chao; Tang, Tang; Chen, Chinpan. "Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein." Proteins 78, 2202-2212 (2010).
Assembly members:
mTZD_finger1, polymer, 28 residues, 3339.8 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
mTZD_finger1: MRPYSCSVCGKRFSLKHQME
THYRVHTG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 110 |
15N chemical shifts | 26 |
1H chemical shifts | 168 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | zinc finger protein | 1 |
2 | ZINC ION | 2 |
Entities:
Entity 1, zinc finger protein 28 residues - 3339.8 Da.
1 | MET | ARG | PRO | TYR | SER | CYS | SER | VAL | CYS | GLY | ||||
2 | LYS | ARG | PHE | SER | LEU | LYS | HIS | GLN | MET | GLU | ||||
3 | THR | HIS | TYR | ARG | VAL | HIS | THR | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: acetic acid, [U-99% 2H], 50 mM; sodium chloride 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR v2.18, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMRView v5, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Related Database Links:
PDB | |
GB | AAF61244 AAI37688 AAK13198 EDL24005 EDM07732 |
REF | NP_001102987 NP_001178153 NP_067372 XP_001492305 XP_004015262 |
SP | Q9JKD9 |
TPG | DAA19797 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts