BMRB Entry 16877

Assembly:

Entities:

Entity 1, TAR RNA bulge 29 residues - Formula weight is not available

Entity 2, Tat protein 14 residues - Formula weight is not available

Samples:

sample_1: HIV-1 TAR RNA 1.0 ± 0.1 mM; L-22 peptide 1.0 ± 0.2 mM; D2O 100%; potassium phosphate 10 mM

sample_2: HIV-1 TAR RNA 1.0 ± 0.1 mM; L-22 peptide 1.0 ± 0.2 mM; D2O 10%; potassium phosphate 10 mM; H2O 90%

sample_3: HIV-1 TAR RNA, [U-98% 13C; U-98% 15N], 1.0 ± 0.1 mM; L-22 peptide 1.0 ± 0.2 mM; D2O 100%; potassium phosphate 10 mM

sample_4: HIV-1 TAR RNA, [U-98% 13C; U-98% 15N], 1.0 ± 0.1 mM; L-22 peptide 1.0 ± 0.2 mM; D2O 10%; potassium phosphate 10 mM; H2O 90%

sample_5: HIV-1 TAR RNA, [U-2H], 1.0 ± 0.2 mM; L-22 peptide 1.0 ± 0.2 mM; D2O 100%; potassium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.6; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 10 mM; pH: 6.6; pressure: 1 atm; temperature: 277 K

Experiments:

Software:

X-PLOR NIH v2.16.0, C.D. Schwieters, J.J. Kuszewski, N. Tjandra and G.M. Clore - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - peak picking

NMR spectrometers:

• Bruker DRX 500 MHz
• Bruker DMX 600 MHz
• Varian Inova 800 MHz
• Bruker AVIII 600 MHz