BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16886

Title: NS2 [27-59]   PubMed: 21187906

Deposition date: 2010-04-21 Original release date: 2011-05-19

Authors: Montserret, Roland; Bartenschlager, Ralf; Penin, Francois

Citation: Jirasko, Vlastimil; Montserret, Roland; Lee, Ji Young; Gouttenoire, Jerome; Moradpour, Darius; Penin, Francois; Bartenschlager, Ralf. "Structural and functional studies of nonstructural protein 2 of the hepatitis C virus reveal its key role as organizer of virion assembly."  PLoS Pathog. 6, .-. (2010).

Assembly members:
HCV NS2 [27-59], polymer, 33 residues, 4054.897 Da.

Natural source:   Common Name: Hepatitis C Virus   Taxonomy ID: 11103   Superkingdom: virus   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C Virus

Experimental source:   Production method: chemical synthesis   Host organism: none

Entity Sequences (FASTA):
HCV NS2 [27-59]: KLFLARLIWWLQYFITRAEA HLQVWIPPLNVRG

Data sets:
Data typeCount
13C chemical shifts120
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HCV NS2 [27-59]1

Entities:

Entity 1, HCV NS2 [27-59] 33 residues - 4054.897 Da.

1   LYSLEUPHELEUALAARGLEUILETRPTRP
2   LEUGLNTYRPHEILETHRARGALAGLUALA
3   HISLEUGLNVALTRPILEPROPROLEUASN
4   VALARGGLY

Samples:

sample_1: NS2 1.2 mM; TFE, [U-2H], 50 % v/v; H2O 50 % v/v

sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

SPARKY, Goddard - chemical shift assignment

X-PLOR NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
DBJ BAA01728 BAB18800 BAB18808 BAD73970 BAD73971
EMBL CAB46677 CAB46911 CAB46915
GB AAD56184 AAD56185 AAD56192 AAD56193 AAF65942
SP P29846 Q9WMX2