BMRB Entry 16905
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16905
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Title: Sma0114 PubMed: 20936511
Deposition date: 2010-04-29 Original release date: 2010-10-27
Authors: Sheftic, Sarah; Gage, Dan; Alexandrescu, Andrei
Citation: Sheftic, Sarah; Garcia, Preston; Robinson, Victoria; Gage, Daniel; Alexandrescu, Andrei. "NMR assignments for the Sinorhizobium meliloti response regulator Sma0114." Biomol. NMR Assignments 5, 55-58 (2011).
Assembly members:
Sma0114, polymer, 123 residues, Formula weight is not available
Natural source: Common Name: Sinorhizobium meliloti Taxonomy ID: 382 Superkingdom: Bacteria Kingdom: not available Genus/species: Sinorhizobium meliloti
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Sma0114: GSHMTERRLRVLVVEDESMI
AMLIEDTLCELGHEVAATAS
RMQEALDIARKGQFDIAIID
VNLDGEPSYPVADILAERNV
PFIFATGYGSKGLDTRYSNI
PLLTKPFLDSELEAVLVQIS
KEV
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 478 |
| 15N chemical shifts | 119 |
| 1H chemical shifts | 660 |
| coupling constants | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | response regulator | 1 |
Entities:
Entity 1, response regulator 123 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | THR | GLU | ARG | ARG | LEU | ARG | ||||
| 2 | VAL | LEU | VAL | VAL | GLU | ASP | GLU | SER | MET | ILE | ||||
| 3 | ALA | MET | LEU | ILE | GLU | ASP | THR | LEU | CYS | GLU | ||||
| 4 | LEU | GLY | HIS | GLU | VAL | ALA | ALA | THR | ALA | SER | ||||
| 5 | ARG | MET | GLN | GLU | ALA | LEU | ASP | ILE | ALA | ARG | ||||
| 6 | LYS | GLY | GLN | PHE | ASP | ILE | ALA | ILE | ILE | ASP | ||||
| 7 | VAL | ASN | LEU | ASP | GLY | GLU | PRO | SER | TYR | PRO | ||||
| 8 | VAL | ALA | ASP | ILE | LEU | ALA | GLU | ARG | ASN | VAL | ||||
| 9 | PRO | PHE | ILE | PHE | ALA | THR | GLY | TYR | GLY | SER | ||||
| 10 | LYS | GLY | LEU | ASP | THR | ARG | TYR | SER | ASN | ILE | ||||
| 11 | PRO | LEU | LEU | THR | LYS | PRO | PHE | LEU | ASP | SER | ||||
| 12 | GLU | LEU | GLU | ALA | VAL | LEU | VAL | GLN | ILE | SER | ||||
| 13 | LYS | GLU | VAL |
Samples:
sample_1: Sma114, [U-100% 15N], 500 uM; H2O 90%; D2O 10%; sodium phosphate 50 mM; DTT 1 mM
sample_2: Sma114, [U-100% 13C; U-100% 15N], 500 uM; D2O 100%; sodium phosphate 50 mM; DTT 1 mM
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
| HSQC TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNHB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX, Accelrys Software Inc. - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 19286 |
| PDB | |
| EMBL | CCM71308 CDH82688 |
| GB | AAK64716 AEG06710 AEG57042 AEH82764 AGG69741 |
| REF | NP_435304 WP_010967059 WP_033048070 WP_046066705 |
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