BMRB Entry 16944

Assembly:

Entities:

Entity 1, CpR82G 68 residues - 12660.391 Da.

LEHHHHHH are at the C-terminus as part of a His -Tag. It is not included in the coordinate file.

Samples:

sample_1: CpR82G, [U-100% 13C; U-100% 15N], 1.01 mM; sodium azide 0.02%; DTT 100 mM; Calcium Chloride 5 mM; sodium chloride 100 mM; MES 20 mM; H2O 95%; D2O 5%

sample_2: CpR82G, U-5% 13C; U-100% 15N, 0.98 mM; sodium azide 0.02%; DTT 100 mM; Calcium Chloride 5 mM; sodium chloride 100 mM; MES 20 mM; H2O 95%; D2O 5%

sample_1_Peg: CpR82G, [U-100% 13C; U-100% 15N], 0.67 mM; sodium azide 0.016%; DTT 81.73 mM; Calcium Chloride 4.09 mM; sodium chloride 81.73 mM; MES 16.35 mM; C12E5 PEG 4%; Hexanol 1.25%; H2O 82.88%; D2O 17.12%

sample_1_Gel: CpR82G 0.92 mM; sodium azide 0.018%; DTT 91.67 mM; Calcium Chloride 4.58 mM; sodium chloride 91.67 mM; MES 18.32 mM; Positively Charged Compressed Polyacrylamide Gel 7%; H2O 91.3%; D2O 8.7%

sample_conditions_1: ionic strength: 205 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CYANA v2.1.5, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMRViewJ v8.0.b17, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

PSVS, Bhattacharya and Montelione - data analysis

NMR spectrometers:

• Varian INOVA 600 MHz
• Varian INOVA 600 MHz