BMRB Entry 16954

Name: Structure of the AML1-ETO Nervy Domain - PKA(RIIa) complex and its contribution to AML1-ETO activity
Published: 2010-08-19

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PDB ID: 2kyg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16954
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure of the AML1-ETO Nervy Domain - PKA(RIIa) complex and its contribution to AML1-ETO activity PubMed: 20708017

Deposition date: 2010-05-25 Original release date: 2010-08-19

Authors: Corpora, Takeshi; Cierpecki, Tomasz; Bushweller, John

Citation: Corpora, Takeshi; Roudaia, Liya; Oo, Zaw Min; Chen, Wei; Manuylova, Ekaterina; Cai, Xiongwei; Chen, Michael; Cierpicki, Tomasz; Speck, Nancy; Bushweller, John. "Structure of the AML1-ETO NHR3-PKA(RII) Complex and Its Contribution to AML1-ETO Activity." J. Mol. Biol. 402, 560-577 (2010).

Assembly members:
PKA(RIIa), polymer, 50 residues, 5621.476 Da.
NHR3, polymer, 38 residues, 4184.758 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
PKA(RIIa): GAMGSMSHIQIPPGLTELLQ GYTVEVLRQQPPDLVEFAVE YFTRLREARA
NHR3: AMADIGSASGYVPEEIWKKA EEAVNEVKRQAMTELQKA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	470
15N chemical shifts	134
1H chemical shifts	897

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	PKA(RIIa) 1	1
2	PKA(RIIa) 2	1
3	NHR3	2

Samples:

CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa): NHR3, [U-99% 13C; U-99% 15N], 2 mM; PKA(RIIa) 4 mM; NaPi 20 mM; EDTA 1 mM

CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3: PKA(RIIa), [U-99% 13C; U-99% 15N], 4 mM; NHR3 2 mM; NaPi 20 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.0 M; pH: 4.0; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D CBCA(CO)NH	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D HNCO	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D HNCACB	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D HCCH-TOCSY	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D HNHA	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D H(CCO)NH	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D 1H-15N NOESY	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
3D 1H-13C NOESY	CN-labelled_NHR3_in_complex_with_unlabelled_PKA(RIIa)	isotropic	sample_conditions_1
2D 1H-15N HSQC	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D CBCA(CO)NH	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D HNCO	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D HNCACB	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D HCCH-TOCSY	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D HNHA	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D H(CCO)NH	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D 1H-15N NOESY	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1
3D 1H-13C NOESY	CN-labelled_PKA(RIIa)_in_complex_with_unlabelled_NHR3	isotropic	sample_conditions_1

1	GLY	ALA	MET	GLY	SER	MET	SER	HIS	ILE	GLN
2	ILE	PRO	PRO	GLY	LEU	THR	GLU	LEU	LEU	GLN
3	GLY	TYR	THR	VAL	GLU	VAL	LEU	ARG	GLN	GLN
4	PRO	PRO	ASP	LEU	VAL	GLU	PHE	ALA	VAL	GLU
5	TYR	PHE	THR	ARG	LEU	ARG	GLU	ALA	ARG	ALA

PDB	2HWN 2IZX 2KYG 2KYG
DBJ	BAF85731 BAG73608 BAK63879 BAA03089 BAA03247 BAA03558 BAA06774 BAA07755
EMBL	CAA33094 CAA67817 CAA56311 CAG33073
GB	AAH02763 AAM97689 AAP35889 AAP36931 AAX29035 AAB34819 AAC28931 AAC28932 AAH05850 AAH67078
PRF	1506340A
REF	NP_001266877 NP_004148 NP_062137 XP_003894514 XP_005075011 NP_001102127 NP_001104496 NP_001104497 NP_001185554 NP_001185555
SP	P12368 P13861 Q06455 Q61909

Biological Magnetic Resonance Data Bank

BMRB Entry 16954

Title: Structure of the AML1-ETO Nervy Domain - PKA(RIIa) complex and its contribution to AML1-ETO activity PubMed: 20708017

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	ALA	MET	GLY	SER	MET	SER	HIS	ILE	GLN
2	ILE	PRO	PRO	GLY	LEU	THR	GLU	LEU	LEU	GLN
3	GLY	TYR	THR	VAL	GLU	VAL	LEU	ARG	GLN	GLN
4	PRO	PRO	ASP	LEU	VAL	GLU	PHE	ALA	VAL	GLU
5	TYR	PHE	THR	ARG	LEU	ARG	GLU	ALA	ARG	ALA

1	GLY	ALA	MET	GLY	SER	MET	SER	HIS	ILE	GLN
2	ILE	PRO	PRO	GLY	LEU	THR	GLU	LEU	LEU	GLN
3	GLY	TYR	THR	VAL	GLU	VAL	LEU	ARG	GLN	GLN
4	PRO	PRO	ASP	LEU	VAL	GLU	PHE	ALA	VAL	GLU
5	TYR	PHE	THR	ARG	LEU	ARG	GLU	ALA	ARG	ALA

1	GLY	ALA	MET	GLY	SER	MET	SER	HIS	ILE	GLN
2	ILE	PRO	PRO	GLY	LEU	THR	GLU	LEU	LEU	GLN
3	GLY	TYR	THR	VAL	GLU	VAL	LEU	ARG	GLN	GLN
4	PRO	PRO	ASP	LEU	VAL	GLU	PHE	ALA	VAL	GLU
5	TYR	PHE	THR	ARG	LEU	ARG	GLU	ALA	ARG	ALA