BMRB Entry 16957

Name: Solution structure of the voltage-sensing domain of KvAP
Published: 2010-09-24

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PDB ID: 2kyh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16957
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the voltage-sensing domain of KvAP PubMed: 20851706

Deposition date: 2010-05-26 Original release date: 2010-09-24

Authors: Butterwick, Joel; MacKinnon, Roderick

Citation: Butterwick, Joel; MacKinnon, Roderick. "Solution structure and phospholipid interactions of the isolated voltage-sensor domain from KvAP." J. Mol. Biol. 403, 591-606 (2010).

Assembly members:
KvAP, polymer, 147 residues, 16197.432 Da.

Natural source: Common Name: Aeropyrum pernix Taxonomy ID: 56636 Superkingdom: Archaea Kingdom: not available Genus/species: Aeropyrum pernix

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
KvAP: LRGLSDLGGRVRNIGDVMEH PLVELGVSYAALLSVIVVVV EYTMQLSGEYLVRLYLVDLI LVIILWADYAYRAYKSGDPA GYVKKTLYEIPALVPAGLLA LIEGHLAGLGLFRLVRLLRF LRILLIISRGSKFLSAIADA ADKLVPR

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	353
15N chemical shifts	231
1H chemical shifts	233

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	voltage-sensor domain of KvAP	1

Samples:

sample_N: KvAP, [U-98% 15N], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_Nd2o: KvAP, [U-98% 15N], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; D2O 99%

sample_DCN: KvAP, [U-100% 13C; U-100% 15N; U-50% 2H], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_CN: KvAP, [U-98% 13C; U-98% 15N], 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; D2O 99%

sample_methyl: KvAP, [13CH3/12CD2]-Leu,Val;[13CH3]-Ile, 0.1 – 0.5 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_Arg: KvAP, [U-98% 13C; U-98% 15N]-Arg, 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_LGA: KvAP, [98% 1-13C,98% 15N]-Leu; [98% 2-13C]-Gly; [98% 2,3-13C]-Ala, 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_ILV: KvAP, [98% 15N]-Ile; [98% 2-13C]-Leu; [98% 1-13C]-Val, 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; H2O 90%; D2O 10%

sample_10C: KvAP, [U-10% 13C], 0.3 – 0.3 mM; HEPES 20 mM; potassium chloride 20 mM; diheptanoyl phosphatidylcholine mM; D2O 99%

sample_conditions_1: ionic strength: 40 mM; pH: 7.0; pressure: 1 atm; temperature: 318 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_N	isotropic	sample_conditions_1
3D TROSY HNCO	sample_DCN	isotropic	sample_conditions_1
3D TROSY HNCA	sample_DCN	isotropic	sample_conditions_1
3D TROSY HN(CO)CA	sample_DCN	isotropic	sample_conditions_1
3D HCCH-COSY	sample_CN	isotropic	sample_conditions_1
3D TROSY HNCACB	sample_DCN	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_N	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_CN	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_methyl	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_DCN	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_Nd2o	isotropic	sample_conditions_1
2D HN(CO)	sample_LGA	isotropic	sample_conditions_1
2D H(N)CO	sample_LGA	isotropic	sample_conditions_1
2D HN(CA)	sample_LGA	isotropic	sample_conditions_1
2D H(N)CA	sample_LGA	isotropic	sample_conditions_1
2D HN(CO)	sample_ILV	isotropic	sample_conditions_1
2D H(N)CO	sample_ILV	isotropic	sample_conditions_1
2D HN(CA)	sample_ILV	isotropic	sample_conditions_1
2D H(N)CA	sample_ILV	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_LGA	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_ILV	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_Arg	isotropic	sample_conditions_1
2D HN(CA)	sample_Arg	isotropic	sample_conditions_1
2D H(N)CA	sample_Arg	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_DCN	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_Nd2o	isotropic	sample_conditions_1
3D 1H-13C NOESYaro	sample_CN	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_10C	isotropic	sample_conditions_1

PDB	1ORQ 1ORS 2A0L 2KYH
DBJ	BAA79939
SP	Q9YDF8

Biological Magnetic Resonance Data Bank

BMRB Entry 16957

Title: Solution structure of the voltage-sensing domain of KvAP PubMed: 20851706

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