BMRB Entry 16992
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16992
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Title: 1H,13C,15N backbone and sidechain assignment of human Raf kinase inhibitor protein from Escherichia coli PubMed: 20924725
Deposition date: 2010-06-09 Original release date: 2010-10-14
Authors: Cuiying, Yi
Citation: Yi, Cuiying; Peng, Yu; Guo, Chenyun; Lin, Donghai. "(1)H, ( 13)C, ( 15)N backbone and side-chain resonance assignments of the human Raf-1 kinase inhibitor protein." Biomol. NMR Assignments 5, 63-66 (2011).
Assembly members:
RKIP, polymer, 202 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RKIP: MSYYHHHHHHEGVRTMPVDL
SKWSGPLSLQEVDEQPQHPL
HVTYAGAAVDELGKVLTPTQ
VKNRPTSISWDGLDSGKLYT
LVLTDPDAPSRKDPKYREWH
HFLVVNMKGNDISSGTVLSD
YVGSGPPKGTGLHRYVWLVY
EQDRPLKCDEPILSNRSGDH
RGKFKVASFRKKYELRAPVA
GTCYQAEWDDYVPKLYEQLS
GK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 722 |
15N chemical shifts | 172 |
1H chemical shifts | 1172 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RKIP | 1 |
Entities:
Entity 1, RKIP 202 residues - Formula weight is not available
1 | MET | SER | TYR | TYR | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | GLU | GLY | VAL | ARG | THR | MET | PRO | VAL | ASP | LEU | ||||
3 | SER | LYS | TRP | SER | GLY | PRO | LEU | SER | LEU | GLN | ||||
4 | GLU | VAL | ASP | GLU | GLN | PRO | GLN | HIS | PRO | LEU | ||||
5 | HIS | VAL | THR | TYR | ALA | GLY | ALA | ALA | VAL | ASP | ||||
6 | GLU | LEU | GLY | LYS | VAL | LEU | THR | PRO | THR | GLN | ||||
7 | VAL | LYS | ASN | ARG | PRO | THR | SER | ILE | SER | TRP | ||||
8 | ASP | GLY | LEU | ASP | SER | GLY | LYS | LEU | TYR | THR | ||||
9 | LEU | VAL | LEU | THR | ASP | PRO | ASP | ALA | PRO | SER | ||||
10 | ARG | LYS | ASP | PRO | LYS | TYR | ARG | GLU | TRP | HIS | ||||
11 | HIS | PHE | LEU | VAL | VAL | ASN | MET | LYS | GLY | ASN | ||||
12 | ASP | ILE | SER | SER | GLY | THR | VAL | LEU | SER | ASP | ||||
13 | TYR | VAL | GLY | SER | GLY | PRO | PRO | LYS | GLY | THR | ||||
14 | GLY | LEU | HIS | ARG | TYR | VAL | TRP | LEU | VAL | TYR | ||||
15 | GLU | GLN | ASP | ARG | PRO | LEU | LYS | CYS | ASP | GLU | ||||
16 | PRO | ILE | LEU | SER | ASN | ARG | SER | GLY | ASP | HIS | ||||
17 | ARG | GLY | LYS | PHE | LYS | VAL | ALA | SER | PHE | ARG | ||||
18 | LYS | LYS | TYR | GLU | LEU | ARG | ALA | PRO | VAL | ALA | ||||
19 | GLY | THR | CYS | TYR | GLN | ALA | GLU | TRP | ASP | ASP | ||||
20 | TYR | VAL | PRO | LYS | LEU | TYR | GLU | GLN | LEU | SER | ||||
21 | GLY | LYS |
Samples:
sample_1: acetic acid 200 mM; H2O 95%; D2O 5%; RKIP, [U-100% 13C; U-100% 15N], 3 mM; DTT 5 mM
sample_conditions_1: ionic strength: 0.4 M; pH: 4.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
BMRB | 17382 18204 |
PDB | |
DBJ | BAA03684 BAE88027 BAE88359 BAG34868 BAG61396 |
EMBL | CAA51652 CAA53031 CAA59404 CAH93256 |
GB | AAB32876 AAD14234 AAH08714 AAH17396 AAH31102 |
PRF | 2117380B |
REF | NP_001126915 NP_001233128 NP_002558 XP_002753109 XP_004054017 |
SP | P30086 P48737 Q5R4R0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts