BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17001

Title: ASIP(80-132, P103A, P105A, Q115Y, S124Y)   PubMed: 20831872

Deposition date: 2010-06-14 Original release date: 2010-09-24

Authors: Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-kui; Kaelin, Chris; Barsh, Gregory; Millhauser, Glenn

Citation: Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-Kui; Kaelin, Christopher; Barsh, Gregory; Millhauser, Glenn. "Loop-swapped chimeras of the agouti-related protein and the agouti signaling protein identify contacts required for melanocortin 1 receptor selectivity and antagonism."  J. Mol. Biol. 404, 45-55 (2010).

Assembly members:
ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y), polymer, 53 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y): KKVVRPRTPLSAPCVATRNS CKPAAAACCDPCASCYCRFF RSACYCRVLSLNC

Data sets:
Data typeCount
1H chemical shifts303

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y)1

Entities:

Entity 1, ASIP(80-132,_P103A,_P105A,_Q115Y,_S124Y) 53 residues - Formula weight is not available

1   LYSLYSVALVALARGPROARGTHRPROLEU
2   SERALAPROCYSVALALATHRARGASNSER
3   CYSLYSPROALAALAALAALACYSCYSASP
4   PROCYSALASERCYSTYRCYSARGPHEPHE
5   ARGSERALACYSTYRCYSARGVALLEUSER
6   LEUASNCYS

Samples:

sample_1: ASIP(80-132, P103A, P105A, Q115Y, S124Y) 1-2 mM; D2O 10%; H2O 90%; acetic acid, [U-100% 2H], 50 mM; sodium azide .1 % w/v; TSP 200 uM

sample_2: ASIP(80-132, P103A, P105A, Q115Y, S124Y) 1-2 mM; D2O, [U-100% 2H], 100%; acetic acid, [U-100% 2H], 50 mM; sodium azide .1 % w/v; TSP 200 uM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17086
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