BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17055

Title: Binding Site Structure of One LRP-RAP Complex Implications for a Common Ligand-Receptor Binding Motif   PubMed: 16938309

Deposition date: 2010-07-13 Original release date: 2010-09-08

Authors: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzerodt, Michael; Thogersen, Hans; O'Shea, Charlotte; Poulsen, Flemming; Kragelund, Birthe

Citation: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzerodt, Michael; Thogersen, Hans; O'Shea, Charlotte; Poulsen, Flemming; Kragelund, Birthe. "Binding Site Structure of One LRP-RAP Complex: Implications for a Common Ligand-Receptor Binding Motif"  J. Mol. Biol. 362, 700-716 (2006).

Assembly members:
CR56, polymer, 82 residues, Formula weight is not available
RAPd1, polymer, 81 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CR56: SARTCPPNQFSCASGRCIPI SWTCDLDDDCGDRSDESASC AYPTCFPLTQFTCNNGRCIN INWRCDNDNDCGDNSDEAGC SH
RAPd1: GEEFRMEKLNQLWEKAQRLH LPPVRLAELHADLKIQERDE LAWKKLKLDGLDEDGEKEAR LIRNLNVILAKYGLDGKKDA R

Data sets:
  • assigned_chemical_shifts
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CR561
2RAPd12

Entities:

Entity 1, CR56 82 residues - Formula weight is not available

1   SERALAARGTHRCYSPROPROASNGLNPHE
2   SERCYSALASERGLYARGCYSILEPROILE
3   SERTRPTHRCYSASPLEUASPASPASPCYS
4   GLYASPARGSERASPGLUSERALASERCYS
5   ALATYRPROTHRCYSPHEPROLEUTHRGLN
6   PHETHRCYSASNASNGLYARGCYSILEASN
7   ILEASNTRPARGCYSASPASNASPASNASP
8   CYSGLYASPASNSERASPGLUALAGLYCYS
9   SERHIS

Entity 2, RAPd1 81 residues - Formula weight is not available

1   GLYGLUGLUPHEARGMETGLULYSLEUASN
2   GLNLEUTRPGLULYSALAGLNARGLEUHIS
3   LEUPROPROVALARGLEUALAGLULEUHIS
4   ALAASPLEULYSILEGLNGLUARGASPGLU
5   LEUALATRPLYSLYSLEULYSLEUASPGLY
6   LEUASPGLUASPGLYGLULYSGLUALAARG
7   LEUILEARGASNLEUASNVALILELEUALA
8   LYSTYRGLYLEUASPGLYLYSLYSASPALA
9   ARG

Samples:

sample_1: CR56, [U-15N], 20 uM; RAPd10 – 40 uM; sodium chloride 100 mM; calcium chloride 10 mM; magnesium chloride 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NACCESS, Hubbard, S. J. and Thornton, J. M. (1993) - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PRONTO3D, Kj r, M., Andersen, K. V. & Poulsen, F. M. (1994). - data analysis

NMR spectrometers:

  • Varian Unity 750 MHz
  • Varian Unity 800 MHz

Related Database Links:

PDB
BMRB 16454 5598
DBJ BAD96224 BAD96247 BAE01566 BAE87201 BAF85010
EMBL CAH90295
GB AAA51553 AAC67373 AAI05075 AAI12068 AAY18903
REF NP_001125136 NP_001233320 NP_002328 XP_001085674 XP_003279069
SP P30533