BMRB Entry 17086
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17086
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Title: 1H assignments for ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y) PubMed: 20831872
Deposition date: 2010-07-28 Original release date: 2011-10-28
Authors: Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-kui; Kaelin, Chris; Barsh, Gregory; Millhauser, Glenn
Citation: Patel, Mira; Cribb Fabersunne, Camila; Yang, Ying-Kui; Kaelin, Christopher; Barsh, Gregory; Millhauser, Glenn. "Loop-swapped chimeras of the agouti-related protein and the agouti signaling protein identify contacts required for melanocortin 1 receptor selectivity and antagonism." J. Mol. Biol. 404, 45-55 (2010).
Assembly members:
ASIP(93-126,_P103A,_P105A,_P111A,_Q115Y,_S124Y), polymer, 34 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
ASIP(93-126,_P103A,_P105A,_P111A,_Q115Y,_S124Y): CVATRNSCKPAAAACCDPAA
SCYCRFFRSACYCR
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 182 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y) | 1 |
Entities:
Entity 1, ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y) 34 residues - Formula weight is not available
| 1 | CYS | VAL | ALA | THR | ARG | ASN | SER | CYS | LYS | PRO | ||||
| 2 | ALA | ALA | ALA | ALA | CYS | CYS | ASP | PRO | ALA | ALA | ||||
| 3 | SER | CYS | TYR | CYS | ARG | PHE | PHE | ARG | SER | ALA | ||||
| 4 | CYS | TYR | CYS | ARG |
Samples:
sample_1: ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)1 – 2 mM; D2O 10%; acetic acid, [U-100% 2H], 50 mM; sodium azide 0.1 w/v; TSP 200 uM; H2O 90%
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Varian INOVA 600 MHz