BMRB Entry 17101
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17101
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Title: 1H NMR study of the interaction of N,N',N''-triacetyl chitotriose with Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus PubMed: 7656986
Deposition date: 2010-08-02 Original release date: 2010-09-08
Authors: Verheyden, Patricia; Pletinckx, Jurgen; Maes, Dominique; Pepermans, Henri; Wyns, Lode; Willem, Rudolph; Martins, Jose
Citation: Verheyden, Patricia; Pletinckx, Jurgen; Maes, Dominique; Pepermans, Henri; Wyns, Lode; Willem, Rudolph; Martins, Jose. "1H NMR study of the interaction of N,N',N''-triacetyl chitotriose with Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus" FEBS Lett. 370, 245-249 (1995).
Assembly members:
AMP2, polymer, 30 residues, Formula weight is not available
CTO, non-polymer, 627.593 Da.
Natural source: Common Name: Amaranth Taxonomy ID: 3567 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Amaranthus caudatus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
AMP2: VGECVRGRCPSGMCCSQFGY
CGKGPKYCGR
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | AMP2 | 1 |
2 | CTO | 2 |
Entities:
Entity 1, AMP2 30 residues - Formula weight is not available
1 | VAL | GLY | GLU | CYS | VAL | ARG | GLY | ARG | CYS | PRO | |
2 | SER | GLY | MET | CYS | CYS | SER | GLN | PHE | GLY | TYR | |
3 | CYS | GLY | LYS | GLY | PRO | LYS | TYR | CYS | GLY | ARG |
Entity 2, CTO - C24 H41 N3 O16 - 627.593 Da.
1 | CTO |
Samples:
sample_1: sodium azide 50 uM; Na2HPO4/KH2PO4 buffer 20 mM; AMP2 2.5 mM; CTO0 0.3 M/L; D2O 100%
sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 315 K
sample_conditions_2: pH: 2.4; pressure: 1 atm; temperature: 315 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H NMR | sample_1 | isotropic | sample_conditions_1 |
1H NMR | sample_1 | isotropic | sample_conditions_2 |
Software:
UXNMR, Bruker Biospin - baseline corrections
Sigmaplot v4.1, (Jandel Scientific) - data analysis
NMR spectrometers:
- Bruker AMX 600 MHz