BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17108

Title: NMR STRUCTURE OF HUMAN INSULIN MUTANT ILE-A2-LEU, VAL-A3-LEU 2 HIS-B10-ASP, PRO-B28-LYS, LYS-B29-PRO, 20 STRUCTURES

Deposition date: 2010-08-09 Original release date: 2012-08-02

Authors: Wan, Z.; Hua, Q.; Huang, K.; Hu, S.; Philips, N.; Katsoyannis, J.; Weiss, M.

Citation: Wan, Z.; Hua, Q.; Huang, K.; Hu, S.; Philips, N.; Whittaker, J.; Katsoyannis, P.; Weiss, M.. "Chiral Protein Engineering and its Application in G Health"  J. Biol. Chem. ., .-..

Assembly members:
entity_1, polymer, 21 residues, 2434.771 Da.
Human Insulin, polymer, 30 residues, 3410.921 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GLLEQCCHSICSLYQLENYC N
Human Insulin: FVNQHLCGSDLVEALYLVCG ERGFFYTKPT

Data sets:
Data typeCount
1H chemical shifts347

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Insulin Mutant, 11
2Human Insulin Mutant, 22

Entities:

Entity 1, Human Insulin Mutant, 1 21 residues - 2434.771 Da.

1   GLYLEULEUGLUGLNCYSCYSHISSERILE
2   CYSSERLEUTYRGLNLEUGLUASNTYRCYS
3   ASN

Entity 2, Human Insulin Mutant, 2 30 residues - 3410.921 Da.

1   PHEVALASNGLNHISLEUCYSGLYSERASP
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRLYSPROTHR

Samples:

sample_1: entity_1 0.5 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0 mM; pH: 7.0; pressure: . mbar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1isotropicsample_conditions_1
NOESYsample_1isotropicsample_conditions_1
COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR v3.5, Brunger - structure solution

NMR spectrometers:

  • BRUKER DRX 700 MHz

Related Database Links:

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