BMRB Entry 17132
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17132
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Title: Solution Structure of human UBA-like domain of OTUD7A_11_83, NESG target HT6304a/OCSP target OTUD7A_11_83/SGC-Toronto
Deposition date: 2010-08-17 Original release date: 2010-09-02
Authors: Wu, Bin; Yee, Adelinda; Lemak, Alexander; Gutmanas, Aleksandras; Houliston, Scott; Semesi, Anthony; Dhe-Paganon, Sirano; Arrowsmith, Cheryl
Citation: Wu, Bin; Asinas, Abdalin; Yee, Adelinda; Doherty, Ryan; Lemak, Alexander; Gutmanas, Aleksandras; Houliston, Scott; Semesi, Anthony; Arrowsmith, Cheryl; Dhe-Paganon, Sirano. "The amino-terminal UBA domain of OTUD7A" Not known ., .-..
Assembly members:
OTUD7A_11_83, polymer, 73 residues, 8045.992 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
OTUD7A_11_83: SAECWAALLHDPMTLDMDAV
LSDFVRSTGAEPGLARDLLE
GKNWDLTAALSDYEQLRQVH
TANLPHVFNEGRG
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 302 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 483 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OTUD7A_11_83 | 1 |
Entities:
Entity 1, OTUD7A_11_83 73 residues - 8045.992 Da.
| 1 | SER | ALA | GLU | CYS | TRP | ALA | ALA | LEU | LEU | HIS | ||||
| 2 | ASP | PRO | MET | THR | LEU | ASP | MET | ASP | ALA | VAL | ||||
| 3 | LEU | SER | ASP | PHE | VAL | ARG | SER | THR | GLY | ALA | ||||
| 4 | GLU | PRO | GLY | LEU | ALA | ARG | ASP | LEU | LEU | GLU | ||||
| 5 | GLY | LYS | ASN | TRP | ASP | LEU | THR | ALA | ALA | LEU | ||||
| 6 | SER | ASP | TYR | GLU | GLN | LEU | ARG | GLN | VAL | HIS | ||||
| 7 | THR | ALA | ASN | LEU | PRO | HIS | VAL | PHE | ASN | GLU | ||||
| 8 | GLY | ARG | GLY |
Samples:
sample_1: OTUD7A_11_83, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; zinc sulphate 10 uM; DTT, [U-100% 2H], 10 mM; benzamidine 10 mM; inhibitor cocktail 1 x; H2O 90%; D2O 10%
sample_2: OTUD7A_11_83, [U-10% 13C; U-99% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; zinc sulphate 10 uM; DTT, [U-100% 2H], 10 mM; benzamidine 10 mM; inhibitor cocktail 1 x; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
MDDGUI v1.0, Gutmanas, Arrowsmith - processing
SPARKY v3.95, Goddard - data analysis, peak picking
FMCGUI v2.4, Lemak, Arrowsmith - chemical shift assignment, structure refinement
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment
PSVS, Bhattacharya and Montelione - NMR structure quality assessment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAD23047 CAD23048 |
| GB | AAH35668 AAI69221 AAI72582 AHW56608 EDL07269 |
| REF | NP_001193897 NP_570950 NP_570971 XP_001116351 XP_001492477 |
| SP | Q8R554 Q8TE49 |
| TPG | DAA17546 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts