BMRB Entry 17161

Name: Rna14p/Rna15p heterodimer
Published: 2010-10-26

Click here to enlarge.

PDB ID: 2l9b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17161
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Rna14p/Rna15p heterodimer PubMed: 20967574

Deposition date: 2010-08-31 Original release date: 2010-10-26

Authors: Mackereth, Cameron

Citation: Mackereth, Cameron. "Chemical shift assignments of a minimal Rna14p/Rna15p heterodimer from the yeast cleavage factor IA complex." Biomol. NMR Assignments 5, 93-95 (2011).

Assembly members:
Rna14p, polymer, 53 residues, Formula weight is not available
Rna15p, polymer, 109 residues, Formula weight is not available

Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Rna14p: MKRDSELPTEVLDLLSVIPK RQYFNTNLLDAQKLVNFLND QVEIPTVESTKSG
Rna15p: GHMSNGPDFQNSGNANFLSQ KFPELPSGIDVNINMTTPAM MISSELAKKPKEVQLKFLQK FQEWTRAHPEDAASLLELCP QLSFVTAELLLTNGICKVDD LIPLASRPQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	744
15N chemical shifts	166
1H chemical shifts	1167

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Entity Assembly ID	Entity Name	Entity ID
1	Rna14p	1
2	Rna15p	2

Samples:

sample_1: Rna14p, [U-100% 13C; U-100% 15N; U-70% 2H], 1.0 mM; Rna15p, [U-100% 13C; U-100% 15N; U-70% 2H], 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_2: Rna14p, [U-99% 15N], 0.2-1.0 mM; Rna15p, [U-99% 15N], 0.2-1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_3: Rna14p, [U-99% 15N], 0.2 mM; Rna15p, [U-99% 15N], 0.2 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_4: Rna14p, [U-99% 13C; U-99% 15N], 0.4 mM; Rna15p, [U-99% 13C; U-99% 15N], 0.4 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_5: Rna14p, [U-99% 13C; U-99% 15N], 0.4 mM; Rna15p, [U-99% 13C; U-99% 15N], 0.4 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_6: Rna14p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; Rna15p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_7: Rna14p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; Rna15p, [U-100% 13C; U-100% 15N; U-100% 2H, 1H-methyl Ile(delta 1),Leu,Val], 0.5 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_8: Rna14p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; Rna15p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; H2O 90%; D2O 10%

sample_9: Rna14p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; Rna15p, [U-99% 15N];[U-99% 13C]-Leu, 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_10: Rna14p, [U-10% 13C; U-99% 15N], 1.0 mM; Rna15p, [U-10% 13C; U-99% 15N], 1.0 mM; sodium phosphate 20 mM; Nacl 50 mM; DTT 25 mM; D2O 100%

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HMBC	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_3	isotropic	sample_conditions_1
3D H(CCO)NH	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
2D HBHD	sample_5	isotropic	sample_conditions_1
2D HBHE	sample_5	isotropic	sample_conditions_1
3D HCACO	sample_5	isotropic	sample_conditions_1
3D HC(C)H-TOCSY	sample_5	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_5	isotropic	sample_conditions_1
3D C(CO)NH	sample_6	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_7	isotropic	sample_conditions_1
3D HC(C)H-TOCSY	sample_7	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_7	isotropic	sample_conditions_1
2D HNCO	sample_8	isotropic	sample_conditions_1
3D HCACO	sample_9	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_9	isotropic	sample_conditions_1
3D HC(C)H-TOCSY	sample_9	isotropic	sample_conditions_1
2D 1H-13C HSQC-CT	sample_10	isotropic	sample_conditions_1

PDB	2L9B 2L9B
DBJ	GAA25502 GAA23344
EMBL	CAA89771 CAY81879 CAA96746 CAY79715
GB	AAA21300 AHY76517 AJP40760 AJS61929 AJS62366 AAA34984 AHY79328 EDN61560 EDV10275 EDZ72171
REF	NP_013777 NP_011471
SP	P25298 P25299
TPG	DAA09959 DAA08056

Biological Magnetic Resonance Data Bank

BMRB Entry 17161

Title: Rna14p/Rna15p heterodimer PubMed: 20967574

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: