BMRB Entry 17182
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17182
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Title: Weak Substrate Binding to Transport Proteins Studied by NMR PubMed: 9826601
Deposition date: 2010-09-10 Original release date: 2010-11-10
Authors: Spooner, Paul; O'Reilly, W.; Homans, Steven; Rutherford, Nicholas; Henderson, Peter; Watts, Anthony
Citation: Spooner, Paul; O'Reilly, W.; Homans, Steven; Rutherford, Nicholas; Henderson, Peter; Watts, Anthony. "Weak Substrate Binding to Transport Proteins Studied by NMR" Biophys. J. 75, 2794-2800 (1998).
Assembly members:
FucP, polymer, 438 residues, 47545 Da.
ARA, D-SACCHARIDE, 150.130 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: purified from the natural source
- assigned_chemical_shifts
- chemical_rates
| Data type | Count |
| kinetic rates | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FucP | 1 |
| 2 | arabinose | 2 |
Entities:
Entity 1, FucP 438 residues - 47545 Da.
| 1 | MET | GLY | ASN | THR | SER | ILE | GLN | THR | GLN | SER | ||||
| 2 | TYR | ARG | ALA | VAL | ASP | LYS | ASP | ALA | GLY | GLN | ||||
| 3 | SER | ARG | SER | TYR | ILE | ILE | PRO | PHE | ALA | LEU | ||||
| 4 | LEU | CYS | SER | LEU | PHE | PHE | LEU | TRP | ALA | VAL | ||||
| 5 | ALA | ASN | ASN | LEU | ASN | ASP | ILE | LEU | LEU | PRO | ||||
| 6 | GLN | PHE | GLN | GLN | ALA | PHE | THR | LEU | THR | ASN | ||||
| 7 | PHE | GLN | ALA | GLY | LEU | ILE | GLN | SER | ALA | PHE | ||||
| 8 | TYR | PHE | GLY | TYR | PHE | ILE | ILE | PRO | ILE | PRO | ||||
| 9 | ALA | GLY | ILE | LEU | MET | LYS | LYS | LEU | SER | TYR | ||||
| 10 | LYS | ALA | GLY | ILE | ILE | THR | GLY | LEU | PHE | LEU | ||||
| 11 | TYR | ALA | LEU | GLY | ALA | ALA | LEU | PHE | TRP | PRO | ||||
| 12 | ALA | ALA | GLU | ILE | MET | ASN | TYR | THR | LEU | PHE | ||||
| 13 | LEU | VAL | GLY | LEU | PHE | ILE | ILE | ALA | ALA | GLY | ||||
| 14 | LEU | GLY | CYS | LEU | GLU | THR | ALA | ALA | ASN | PRO | ||||
| 15 | PHE | VAL | THR | VAL | LEU | GLY | PRO | GLU | SER | SER | ||||
| 16 | GLY | HIS | PHE | ARG | LEU | ASN | LEU | ALA | GLN | THR | ||||
| 17 | PHE | ASN | SER | PHE | GLY | ALA | ILE | ILE | ALA | VAL | ||||
| 18 | VAL | PHE | GLY | GLN | SER | LEU | ILE | LEU | SER | ASN | ||||
| 19 | VAL | PRO | HIS | GLN | SER | GLN | ASP | VAL | LEU | ASP | ||||
| 20 | LYS | MET | SER | PRO | GLU | GLN | LEU | SER | ALA | TYR | ||||
| 21 | LYS | HIS | SER | LEU | VAL | LEU | SER | VAL | GLN | THR | ||||
| 22 | PRO | TYR | MET | ILE | ILE | VAL | ALA | ILE | VAL | LEU | ||||
| 23 | LEU | VAL | ALA | LEU | LEU | ILE | MET | LEU | THR | LYS | ||||
| 24 | PHE | PRO | ALA | LEU | GLN | SER | ASP | ASN | HIS | SER | ||||
| 25 | ASP | ALA | LYS | GLN | GLY | SER | PHE | SER | ALA | SER | ||||
| 26 | LEU | SER | ARG | LEU | ALA | ARG | ILE | ARG | HIS | TRP | ||||
| 27 | ARG | TRP | ALA | VAL | LEU | ALA | GLN | PHE | CYS | TYR | ||||
| 28 | VAL | GLY | ALA | GLN | THR | ALA | CYS | TRP | SER | TYR | ||||
| 29 | LEU | ILE | ARG | TYR | ALA | VAL | GLU | GLU | ILE | PRO | ||||
| 30 | GLY | MET | THR | ALA | GLY | PHE | ALA | ALA | ASN | TYR | ||||
| 31 | LEU | THR | GLY | THR | MET | VAL | CYS | PHE | PHE | ILE | ||||
| 32 | GLY | ARG | PHE | THR | GLY | THR | TRP | LEU | ILE | SER | ||||
| 33 | ARG | PHE | ALA | PRO | HIS | LYS | VAL | LEU | ALA | ALA | ||||
| 34 | TYR | ALA | LEU | ILE | ALA | MET | ALA | LEU | CYS | LEU | ||||
| 35 | ILE | SER | ALA | PHE | ALA | GLY | GLY | HIS | VAL | GLY | ||||
| 36 | LEU | ILE | ALA | LEU | THR | LEU | CYS | SER | ALA | PHE | ||||
| 37 | MET | SER | ILE | GLN | TYR | PRO | THR | ILE | PHE | SER | ||||
| 38 | LEU | GLY | ILE | LYS | ASN | LEU | GLY | GLN | ASP | THR | ||||
| 39 | LYS | TYR | GLY | SER | SER | PHE | ILE | VAL | MET | THR | ||||
| 40 | ILE | ILE | GLY | GLY | GLY | ILE | VAL | THR | PRO | VAL | ||||
| 41 | MET | GLY | PHE | VAL | SER | ASP | ALA | ALA | GLY | ASN | ||||
| 42 | ILE | PRO | THR | ALA | GLU | LEU | ILE | PRO | ALA | LEU | ||||
| 43 | CYS | PHE | ALA | VAL | ILE | PHE | ILE | PHE | ALA | ARG | ||||
| 44 | PHE | ARG | SER | GLN | THR | ALA | THR | ASN |
Entity 2, arabinose - 150.130 Da.
| 1 | ARA |
Samples:
sample_1: arabinose, [U-13C], 150.13 ug; FucP 9.5 mg; D-fucose164.2 – 492.5 ug
sample_conditions_1: pH: 7.5; pressure: ambient atm; temperature: 275 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 13C CP MAS | sample_1 | anisotropic | sample_conditions_1 |
Software:
software, na - na
NMR spectrometers:
- Bruker MSL 400 MHz