BMRB Entry 17219
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17219
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Title: Structural Insights into the Monosaccharide Specificity of Escherichia coli Rhamnose Mutarotase PubMed: 15876375
Deposition date: 2010-09-29 Original release date: 2010-11-10
Authors: Ryu, Kyoung-Seok; Kim, Jung-In; Cho, Sung-Jae; Park, Dongkyu; Park, Chankyu; Cheong, Hae-Kap; Lee, Jie-Oh; Choi, Byong-Seok
Citation: Ryu, Kyoung-Seok; Kim, Jung-In; Cho, Sung-Jae; Park, Dongkyu; Park, Chankyu; Cheong, Hae-Kap; Lee, Jie-Oh; Choi, Byong-Seok. "Structural Insights into the Monosaccharide Specificity of Escherichia coli Rhamnose Mutarotase" J. Mol. Biol. 349, 153-162 (2005).
Assembly members:
YiiL, polymer, 104 residues, 164.156 Da.
RNS, non-polymer, 164.156 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
YiiL: MIRKAFVMQVNPDAHEEYQR
RHNPIWPELEAVLKSHGAHN
YAIYLDKARNLLFAMVEIES
EERWNAVASTDVCQRWWKYM
TDVMPANPDNSPVSSELQEV
FYLP
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | YiiL_1 | 1 |
2 | YiiL_2 | 1 |
3 | RNS | 2 |
Entities:
Entity 1, YiiL_1 104 residues - 164.156 Da.
1 | MET | ILE | ARG | LYS | ALA | PHE | VAL | MET | GLN | VAL | ||||
2 | ASN | PRO | ASP | ALA | HIS | GLU | GLU | TYR | GLN | ARG | ||||
3 | ARG | HIS | ASN | PRO | ILE | TRP | PRO | GLU | LEU | GLU | ||||
4 | ALA | VAL | LEU | LYS | SER | HIS | GLY | ALA | HIS | ASN | ||||
5 | TYR | ALA | ILE | TYR | LEU | ASP | LYS | ALA | ARG | ASN | ||||
6 | LEU | LEU | PHE | ALA | MET | VAL | GLU | ILE | GLU | SER | ||||
7 | GLU | GLU | ARG | TRP | ASN | ALA | VAL | ALA | SER | THR | ||||
8 | ASP | VAL | CYS | GLN | ARG | TRP | TRP | LYS | TYR | MET | ||||
9 | THR | ASP | VAL | MET | PRO | ALA | ASN | PRO | ASP | ASN | ||||
10 | SER | PRO | VAL | SER | SER | GLU | LEU | GLN | GLU | VAL | ||||
11 | PHE | TYR | LEU | PRO |
Entity 2, RNS - C6 H12 O5 - 164.156 Da.
1 | RNS |
Samples:
sample_1: YiiL, [U-15N], 1 mM; RNS0 100 mM; D2O 100%
sample_conditions_1: pH: 7.5; pressure: ambient atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
software, na - na
NMR spectrometers:
- Varian Unity Inova 600 MHz