BMRB Entry 17221
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17221
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Title: Basic Requirements for a Metal-Binding Site in a Protein: The Influence of Loop Shortening on the Cupredoxin Azurin PubMed: 16651527
Deposition date: 2010-09-29 Original release date: 2010-11-10
Authors: Li, Chan; Yanagisawa, Sachiko; Martins, Berta; Messerschmidt, Albrecht; Banfield, Mark; Dennison, Christopher
Citation: Li, Chan; Yanagisawa, Sachiko; Martins, Berta; Messerschmidt, Albrecht; Banfield, Mark. "Basic Requirements for a metal-binding site in a protein: The influence of loop shortening on the cupredoxin azurin" Proc. Natl. Acad. Sci. U.S.A. 103, 7258-7263 (2006).
Assembly members:
AZAMIF, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
AZAMIF: AECSVDIQGNDQMQFNTNAI
TVDKSCKQFTVNLSHPGNLP
KNVMGHNWVLSTAADMQGVV
TDGMASGLDKDYLKPDDSRV
IAHTKLIGSGEKDSVTFDVS
KLKEGEQYMFFCTPHPMKGT
LTLK
- assigned_chemical_shifts
- chemical_rates
| Data type | Count |
| kinetic rates | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AZAMIF | 1 |
Entities:
Entity 1, AZAMIF 124 residues - Formula weight is not available
| 1 | ALA | GLU | CYS | SER | VAL | ASP | ILE | GLN | GLY | ASN | ||||
| 2 | ASP | GLN | MET | GLN | PHE | ASN | THR | ASN | ALA | ILE | ||||
| 3 | THR | VAL | ASP | LYS | SER | CYS | LYS | GLN | PHE | THR | ||||
| 4 | VAL | ASN | LEU | SER | HIS | PRO | GLY | ASN | LEU | PRO | ||||
| 5 | LYS | ASN | VAL | MET | GLY | HIS | ASN | TRP | VAL | LEU | ||||
| 6 | SER | THR | ALA | ALA | ASP | MET | GLN | GLY | VAL | VAL | ||||
| 7 | THR | ASP | GLY | MET | ALA | SER | GLY | LEU | ASP | LYS | ||||
| 8 | ASP | TYR | LEU | LYS | PRO | ASP | ASP | SER | ARG | VAL | ||||
| 9 | ILE | ALA | HIS | THR | LYS | LEU | ILE | GLY | SER | GLY | ||||
| 10 | GLU | LYS | ASP | SER | VAL | THR | PHE | ASP | VAL | SER | ||||
| 11 | LYS | LEU | LYS | GLU | GLY | GLU | GLN | TYR | MET | PHE | ||||
| 12 | PHE | CYS | THR | PRO | HIS | PRO | MET | LYS | GLY | THR | ||||
| 13 | LEU | THR | LEU | LYS |
Samples:
sample_1: phosphate10 – 100 mM; AZAMIF 1:1 ratio; D2O 99.9%
sample_conditions_1: pH: 8.0; pressure: ambient atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H NMR | sample_1 | isotropic | sample_conditions_1 |
Software:
PyMol, D eLano Scientific, San Carlos, CA - visualization
NMR spectrometers:
- JEOL lambda 500 MHz
Related Database Links:
| PDB |