BMRB Entry 17276
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17276
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Title: Unbound TbRI-ED PubMed: 21821041
Deposition date: 2010-11-02 Original release date: 2011-10-28
Authors: Zuniga, Jorge; Ilangovan, Udayar; Pardeep, Mahlawat; Hinck, Cynthia; Huang, Tao
Citation: Zuniga, Jorge; Ilangovan, Udayar; Mahlawat, Pardeep; Hinck, Cynthia; Huang, Tao; Groppe, Jay; McEwen, Donald; Hinck, Andrew. "The TR-I pre-helix extension is structurally ordered in the unbound form and its flanking prolines are essential for binding." J. Mol. Biol. 412, 601-618 (2011).
Assembly members:
TbRI, polymer, 87 residues, 9465.8 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TbRI: GSATALQCFCHLCTKDNFTC
VTDGLCFVSVTETTDKVIHN
SMCIAEIDLIPRDRPFVCAP
SSKTGSVTTTYCCNQDHCNK
IELPTTV
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 247 |
15N chemical shifts | 85 |
1H chemical shifts | 529 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unbound Alk5 ectodomain | 1 |
Entities:
Entity 1, unbound Alk5 ectodomain 87 residues - 9465.8 Da.
1 | GLY | SER | ALA | THR | ALA | LEU | GLN | CYS | PHE | CYS | ||||
2 | HIS | LEU | CYS | THR | LYS | ASP | ASN | PHE | THR | CYS | ||||
3 | VAL | THR | ASP | GLY | LEU | CYS | PHE | VAL | SER | VAL | ||||
4 | THR | GLU | THR | THR | ASP | LYS | VAL | ILE | HIS | ASN | ||||
5 | SER | MET | CYS | ILE | ALA | GLU | ILE | ASP | LEU | ILE | ||||
6 | PRO | ARG | ASP | ARG | PRO | PHE | VAL | CYS | ALA | PRO | ||||
7 | SER | SER | LYS | THR | GLY | SER | VAL | THR | THR | THR | ||||
8 | TYR | CYS | CYS | ASN | GLN | ASP | HIS | CYS | ASN | LYS | ||||
9 | ILE | GLU | LEU | PRO | THR | THR | VAL |
Samples:
sample_1: TbRI, [U-100% 13C; U-100% 15N], 1 mM; H2O 95%; D2O 5%; Sodium phosphate 25 mM
sample_conditions_1: ionic strength: 0.025 M; pH: 7.2; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
PDB | |
DBJ | BAD91020 BAD91021 BAD91022 BAD91023 BAG10679 |
EMBL | CAF02096 CAF02097 |
GB | AAA16073 AAA80265 AAC08998 AAD02042 AAH71181 |
REF | NP_001033728 NP_001124388 NP_001293139 NP_004603 XP_001112590 |
SP | P36897 Q5CD18 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts