BMRB Entry 17301
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17301
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Title: NMR Structure of an Archaeal DNA Binding Protein Sso7c4 PubMed: 21546550
Deposition date: 2010-11-16 Original release date: 2011-06-01
Authors: Hsu, Chun-Hua; Wang, Andrew
Citation: Hsu, Chun-Hua; Wang, Andrew H-J. "The DNA-recognition fold of Sso7c4 suggests a new member of SpoVT-AbrB superfamily from archaea." Nucleic Acids Res. 39, 6764-6774 (2011).
Assembly members:
Sso7c4, polymer, 53 residues, 6142 Da.
Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Sso7c4: AVEEIVKVSRNYQVTIPAKV
RQKFQIKEGDLVKVTFDESE
GVVKIQLLKEPWK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 415 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sso7c4, Subunit 1 | 1 |
| 2 | Sso7c4, Subunit 2 | 1 |
Entities:
Entity 1, Sso7c4, Subunit 1 53 residues - 6142 Da.
| 1 | ALA | VAL | GLU | GLU | ILE | VAL | LYS | VAL | SER | ARG | ||||
| 2 | ASN | TYR | GLN | VAL | THR | ILE | PRO | ALA | LYS | VAL | ||||
| 3 | ARG | GLN | LYS | PHE | GLN | ILE | LYS | GLU | GLY | ASP | ||||
| 4 | LEU | VAL | LYS | VAL | THR | PHE | ASP | GLU | SER | GLU | ||||
| 5 | GLY | VAL | VAL | LYS | ILE | GLN | LEU | LEU | LYS | GLU | ||||
| 6 | PRO | TRP | LYS |
Samples:
sample_1: Sso7c4, [U-99% 15N], 1.5 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%
sample_2: Sso7c4 1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.05 M; pH: 5.0; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.2, Goddard - chemical shift assignment
TOPSPIN v2.1, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| GB | ACP36221 ACP38810 ACP46446 ACP47847 ACP56015 |
| REF | WP_012712036 WP_014511480 WP_029552626 |
| SP | P81552 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts