BMRB Entry 17311
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PDB ID: 2lf1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17311
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Title: L.casei DHFR-NADPH complex PubMed: 21410224
Deposition date: 2010-11-22 Original release date: 2011-03-30
Authors: Polshakov, Vladimir; Birdsall, Berry; Feeney, James; Kovalevskaya, Nadezhda
Citation: Feeney, James; Birdsall, Berry; Kovalevskaya, Nadezhda; Smurnyy, Yegor; Navarro Peran, Emna; Polshakov, Vladimir. "NMR Structures of Apo L. casei Dihydrofolate Reductase and Its Complexes with Trimethoprim and NADPH: Contributions to Positive Cooperative Binding from Ligand-Induced Refolding, Conformational Changes, and Interligand Hydrophobic Interactions." Biochemistry 50, 3609-3620 (2011).
Assembly members:
DHFR, polymer, 162 residues, Formula weight is not available
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, non-polymer, 745.421 Da.
Natural source: Common Name: Lactobacillus casei Taxonomy ID: 1582 Superkingdom: Bacteria Kingdom: not available Genus/species: Lactobacillus casei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
DHFR: TAFLWAQDRDGLIGKDGHLP
WHLPDDLHYFRAQTVGKIMV
VGRRTYESFPKRPLPERTNV
VLTHQEDYQAQGAVVVHDVA
AVFAYAKQHPDQELVIAGGA
QIFTAFKDDVDTLLVTRLAG
SFEGDTKMIPLNWDDFTKVS
SRTVEDTNPALTHTYEVWQK
KA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 592 |
| 15N chemical shifts | 173 |
| 1H chemical shifts | 1131 |
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