BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17352

Title: The structure of a domain from yeast   PubMed: 21355849

Deposition date: 2010-12-08 Original release date: 2011-03-30

Authors: Zhang, Wen; Zhang, Jiahai; Tu, Xiaoming

Citation: Zhang, Wen; Zhang, Jiahai; Zhang, Xuecheng; Xu, Chao; Tu, Xiaoming. "Solution structure of the Taf14 YEATS domain and its roles in cell growth of Saccharomyces cerevisiae."  Biochem. J. 436, 83-90 (2011).

Assembly members:
a_domain, polymer, 131 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
a_domain: MVATVKRTIRIKTQQHILPE VPPVENFPVRQWSIEIVLLD DEGKEIPATIFDKVIYHLHP TFANPNRTFTDPPFRIEEQG WGGFPLDISVFLLEKAGERK IPHDLNFLQESYEVEHVIQI PLNLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts107
1H chemical shifts689

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Taf14 YEATS domain1

Entities:

Entity 1, Taf14 YEATS domain 131 residues - Formula weight is not available

1   METVALALATHRVALLYSARGTHRILEARG
2   ILELYSTHRGLNGLNHISILELEUPROGLU
3   VALPROPROVALGLUASNPHEPROVALARG
4   GLNTRPSERILEGLUILEVALLEULEUASP
5   ASPGLUGLYLYSGLUILEPROALATHRILE
6   PHEASPLYSVALILETYRHISLEUHISPRO
7   THRPHEALAASNPROASNARGTHRPHETHR
8   ASPPROPROPHEARGILEGLUGLUGLNGLY
9   TRPGLYGLYPHEPROLEUASPILESERVAL
10   PHELEULEUGLULYSALAGLYGLUARGLYS
11   ILEPROHISASPLEUASNPHELEUGLNGLU
12   SERTYRGLUVALGLUHISVALILEGLNILE
13   PROLEUASNLEUGLUHISHISHISHISHIS
14   HIS

Samples:

sample_1: a_domain 0.7 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_2: a_domain 0.7 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O 100%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, chemical shift calculation, data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
DBJ GAA26845
EMBL CAA49192 CAA81125 CAY86832
GB AAA61644 AAB68235 AHY78050 AJP42018 AJU23462
REF NP_015196
SP P35189
TPG DAA11304

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts