BMRB Entry 17370

Name: Solution NMR structure of conjugate transposon protein BVU_1572(27-141) from Bacteroides Vulgatus, Northeast Structural Genomics Consortium Target BvR155.
Published: 2011-01-05

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PDB ID: 2l7q
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17370
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution NMR structure of conjugate transposon protein BVU_1572(27-141) from Bacteroides Vulgatus, Northeast Structural Genomics Consortium Target BvR155.

Deposition date: 2010-12-20 Original release date: 2011-01-05

Authors: Yang, Yunhuang; Ramelot, Theresa; Cort, John; Wang, Dongyan; Ciccosanti, Colleen; Janjua, Haleema; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael

Citation: Yang, Yunhuang; Ramelot, Theresa; Cort, John; Wang, Dongyan; Ciccosanti, Colleen; Janjua, Haleema; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael. "Solution NMR structure of conjugate transposon protein BVU_1572(27-141) from Bacteroides Vulgatus, Northeast Structural Genomics Consortium Target BvR155." Not known ., .-..

Assembly members:
protein_BVU, polymer, 248 residues, 14645.2 Da.

Natural source: Common Name: Bacteroides Vulgatus Taxonomy ID: 821 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacteroides Vulgatus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
protein_BVU: MNELDIQQEYPFTVESMPVA DEIAGDETVEIRLEIKPSGN FIGTVYTLRYFQPDGKGSLK MEDGTVLKPNDRYLLNEWKF RLYYTSQSDKEAQTIDLYFE DNWGNLQQLTYDFNGKLEHH HHHHMNELDIQQEYPFTVES MPVADEIAGDETVEIRLEIK PSGNFIGTVYTLRYFQPDGK GSLKMEDGTVLKPNDRYLLN EWKFRLYYTSQSDKEAQTID LYFEDNWGNLQQLTYDFNGK LEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	549
15N chemical shifts	132
1H chemical shifts	858

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	protein BVU 1572(27-141)	1

Samples:

NC_sample: conjugate transposon protein BVU_1572(27-141), [U-100% 13C; U-100% 15N], 0.53 ± 0.05 mM; MES 20 ± 1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.25 mM; sodium azide 0.02 ± 0.001 mM; DTT 10 ± 0.5 mM; D2O 10%; H2O 90%

NC5_sample: conjugate transposon protein BVU_1572(27-141), [U-5% 13C; U-100% 15N], 0.48 ± 0.05 mM; MES 20 ± 1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.25 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 mM; D2O 10%; H2O 90%

NC_sample_in_D2O: conjugate transposon protein BVU_1572(27-141), [U-100% 13C; U-100% 15N], 0.53 ± 0.05 mM; MES 20 ± 1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.25 mM; sodium azide 0.02 ± 0.001 %; DTT 10 ± 0.5 mM; D2O 100%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	NC_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC-CT	NC5_sample	isotropic	sample_conditions_1
3D 1H-15N NOESY	NC5_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY-aliph	NC_sample	isotropic	sample_conditions_1
3D HNCO	NC_sample	isotropic	sample_conditions_1
3D HNCACB	NC_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HNCA	NC_sample	isotropic	sample_conditions_1
3D HN(CO)CA	NC_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D H(CCO)NH	NC_sample	isotropic	sample_conditions_1
3D C(CCO)NH	NC_sample	isotropic	sample_conditions_1
3D HCCH-COSY	NC_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	NC_sample	isotropic	sample_conditions_1
3D CCH-TOCSY	NC_sample_in_D2O	isotropic	sample_conditions_1
4D CC-NOESY	NC_sample_in_D2O	isotropic	sample_conditions_1
3D 1H-13C HSQC-aromatic	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	NC_sample_in_D2O	isotropic	sample_conditions_1
3D HCCH-COSY	NC_sample_in_D2O	isotropic	sample_conditions_1
3D HNHA	NC5_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC 150ppm	NC_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2 only	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic no CT	NC_sample	isotropic	sample_conditions_1

Biological Magnetic Resonance Data Bank

BMRB Entry 17370

Title: Solution NMR structure of conjugate transposon protein BVU_1572(27-141) from Bacteroides Vulgatus, Northeast Structural Genomics Consortium Target BvR155.

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