BMRB Entry 17380
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17380
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Title: rbp56 RanBP2 zinc finger backbone chemical shifts PubMed: 21256132
Deposition date: 2010-12-23 Original release date: 2011-01-25
Authors: Mansfield, Robyn; Mackay, Joel; Nguyen, Cuong
Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA." J. Mol. Biol. 407, 273-283 (2011).
Assembly members:
RBP56_ZnF, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RBP56_ZnF: GSRGGDPKSGDWVCPNPSCG
NMNFARRNSCNQCNEPRPED
SR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 74 |
| 15N chemical shifts | 32 |
| 1H chemical shifts | 32 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein component | 1 |
| 2 | zinc ion | 2 |
Entities:
Entity 1, protein component 42 residues - Formula weight is not available
N-terminal GS is non-native, resulting from cleavage of a GST tag.
| 1 | GLY | SER | ARG | GLY | GLY | ASP | PRO | LYS | SER | GLY | ||||
| 2 | ASP | TRP | VAL | CYS | PRO | ASN | PRO | SER | CYS | GLY | ||||
| 3 | ASN | MET | ASN | PHE | ALA | ARG | ARG | ASN | SER | CYS | ||||
| 4 | ASN | GLN | CYS | ASN | GLU | PRO | ARG | PRO | GLU | ASP | ||||
| 5 | SER | ARG |
Entity 2, zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: RBP56 ZnF, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; DSS 20 uM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.125 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 19561 |
| DBJ | BAA33811 BAA33812 BAB46889 BAC34676 BAE27086 |
| EMBL | CAA67398 |
| GB | AAC50932 AAH46099 AAI37592 AAI66769 AAO13485 |
| REF | NP_001099294 NP_001302693 NP_003478 NP_081703 NP_631961 |
| SP | Q92804 |
| TPG | DAA19087 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts