BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17386

Title: RBM10 RanBP2 zinc finger backbone chemical shifts   PubMed: 21256132

Deposition date: 2010-12-23 Original release date: 2011-01-28

Authors: Mansfield, Robyn; Mackay, Joel; Nguyen, Cuong

Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA."  J. Mol. Biol. 407, 273-283 (2011).

Assembly members:
RBM10_ZnF, polymer, 41 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RBM10_ZnF: GSSDPKPKINEDWLCNKCGV QNFKRREKCFKCGVPKSEAE Q

Data sets:
Data typeCount
13C chemical shifts76
15N chemical shifts33
1H chemical shifts33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein component1
2zinc ion2

Entities:

Entity 1, protein component 41 residues - Formula weight is not available

N-terminal GS is non-native, resulting from cleavage of a GST tag.

1   GLYSERSERASPPROLYSPROLYSILEASN
2   GLUASPTRPLEUCYSASNLYSCYSGLYVAL
3   GLNASNPHELYSARGARGGLULYSCYSPHE
4   LYSCYSGLYVALPROLYSSERGLUALAGLU
5   GLN

Entity 2, zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RBM10 ZnF, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; DSS 20 uM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.125 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 25433
PDB
DBJ BAA09471 BAA12144 BAC31087 BAC40753 BAC65490
EMBL CAD97933 CAL37951
GB AAH00681 AAH03089 AAH04181 AAH04674 AAH08733
REF NP_001161247 NP_001161248 NP_001191395 NP_001191396 NP_001191397
SP P70501 P98175 Q99KG3
TPG DAA12902 DAA12903

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts