BMRB Entry 17412

Name: GABARAPL-1 NBR1-LIR complex structure
Published: 2011-06-02

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PDB ID: 2l8j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17412
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: GABARAPL-1 NBR1-LIR complex structure PubMed: 21620860

Deposition date: 2011-01-17 Original release date: 2011-06-02

Authors: Rogov, Vladimir; Rozenknop, Alexis; Rogova, Natalia; Loehr, Frank; Guentert, Peter; Dikic, Ivan; Doetsch, Volker

Citation: Rozenknop, Alexis; Rogov, Vladimir; Rogova, Natalia Yu; Lohr, Frank; Guntert, Peter; Dikic, Ivan; Dotsch, Volker. "Characterization of the Interaction of GABARAPL-1 with the LIR Motif of NBR1." J. Mol. Biol. 410, 477-487 (2011).

Assembly members:
GABARAPL-1, polymer, 119 residues, 14268.327 Da.
NBR1-LIR, polymer, 18 residues, 1840.027 Da.

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
GABARAPL-1: GSPEFKFQYKEDHPFEYRKK EGEKIRKKYPDRVPVIVEKA PKARVPDLDKRKYLVPSDLT VGQFYFLIRKRIHLRPEDAL FFFVNNTIPPTSATMGQLYE DNHEEDYFLYVAYSDESVY
NBR1-LIR: GAMGSASSEDYIIILPES

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	506
15N chemical shifts	110
1H chemical shifts	881

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	GABARAPL-1	1
2	NBR1-LIR	2

Samples:

sample_1: GABARAPL-1, [U-98% 15N], 0.6 ± 0.05 mM; NBR1-LIR 0.9 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%

sample_2: GABARAPL-1, [U-98% 13C; U-98% 15N], 0.6 ± 0.05 mM; NBR1-LIR 0.9 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%

sample_3: GABARAPL-1 0.9 ± 0.05 mM; NBR1-LIR, [U-98% 15N], 0.6 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%

sample_4: GABARAPL-1 0.9 ± 0.05 mM; NBR1-LIR, [U-98% 13C; U-98% 15N], 0.6 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
TROSY-H(CCCO)NH-TOCSY	sample_2	isotropic	sample_conditions_1
(H)CC(CO)NH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_4	isotropic	sample_conditions_1
3D HNCACB	sample_4	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
3D H(C)CH-TOCSY	sample_4	isotropic	sample_conditions_1
TROSY-H(CCCO)NH-TOCSY	sample_4	isotropic	sample_conditions_1
(H)CC(CO)NH-TOCSY	sample_4	isotropic	sample_conditions_1

PDB	2L8J 2R2Q 2L8J
DBJ	BAA95100 BAB27950 BAB29146 BAB29690 BAB31345
EMBL	CAB66611 CAG38511 CAH89636 CAL38063 CAL38067
GB	AAH04602 AAH09309 AAH24706 AAH28315 AAH72921
REF	NP_001025652 NP_001028788 NP_001037759 NP_001085553 NP_001088067
SP	P60518 Q0VGK0 Q5BIZ2 Q5RF21 Q6GQ27
TPG	DAA29267

Biological Magnetic Resonance Data Bank

BMRB Entry 17412

Title: GABARAPL-1 NBR1-LIR complex structure PubMed: 21620860

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