BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17413

Title: B. ste. A26I Holo TRAP Ile, Leu, and Val methyls   PubMed: 22115774

Deposition date: 2011-01-17 Original release date: 2012-02-13

Authors: Kleckner, Ian

Citation: Kleckner, Ian; Gollnick, Paul; Foster, Mark. "Mechanisms of allosteric gene regulation by NMR quantification of microsecond-millisecond protein dynamics"  J. Mol. Biol. 415, 372-381 (2012).

Assembly members:
TRAP, polymer, 74 residues, Formula weight is not available
TRP, non-polymer, 204.225 Da.

Natural source:   Common Name: Bacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TRAP: MYTNSDFVVIKALEDGVNVI GLTRGIDTRFHHSEKLDKGE VLIAQFTEHTSAIKVRGKAY IQTRHGVIESEGKK

Data sets:
Data typeCount
13C chemical shifts29
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRAP, chain 11
2TRAP, chain 21
3TRAP, chain 31
4TRAP, chain 41
5TRAP, chain 51
6TRAP, chain 61
7TRAP, chain 71
8TRAP, chain 81
9TRAP, chain 91
10TRAP, chain 101
11TRAP, chain 111
12L-tryptophan2

Entities:

Entity 1, TRAP, chain 1 74 residues - Formula weight is not available

1   METTYRTHRASNSERASPPHEVALVALILE
2   LYSALALEUGLUASPGLYVALASNVALILE
3   GLYLEUTHRARGGLYILEASPTHRARGPHE
4   HISHISSERGLULYSLEUASPLYSGLYGLU
5   VALLEUILEALAGLNPHETHRGLUHISTHR
6   SERALAILELYSVALARGGLYLYSALATYR
7   ILEGLNTHRARGHISGLYVALILEGLUSER
8   GLUGLYLYSLYS

Entity 2, L-tryptophan - C11 H12 N2 O2 - 204.225 Da.

1   TRP

Samples:

A26I-TRAP-ILV: TRAP, [U-2H; U-15N; ILV-(13CH3)], 1-1.5 mM; potassium chloride 50 mM; sodium chloride 100 mM; sodium azide 0.02%

25C: pH: 8.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticA26I-TRAP-ILVisotropic25C

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 17400
PDB
DBJ BAD76498 GAD12442 GAJ58441
GB AAD33793 ABO67499 ACX77149 ADI26315 ADU94597
REF WP_008879621 WP_011231698 WP_033013997
SP Q9X6J6