BMRB Entry 17414
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PDB ID: 2l8l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17414
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Title: Structure of an engineered splicing intein mutant based on Mycobacterium tuberculosis RecA PubMed: 21604815
Deposition date: 2011-01-19 Original release date: 2012-01-04
Authors: Du, Zhenming; Wang, Chunyu
Citation: Du, Zhenming; Zheng, Yuchuan; Liu, Yangzhong; Belfort, Marlene; Wang, Chunyu. "pK(a) coupling at the intein active site: implications for the coordination mechanism of protein splicing with a conserved aspartate" J. Am. Chem. Soc. 133, 10275-10282 (2011).
Assembly members:
RecA, polymer, 139 residues, 15365.621 Da.
Natural source: Common Name: high GC Gram+ Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RecA: CLAEGTRIFDPVTGTTHRIE
DVVGGRKPIHVVAAAKDGTL
HARPVVSWFDQGTRDVIGLR
IAGGAILWATPDHKVLTEYG
WRAAGELRKGDRVAVRDVET
GELRYSVIREVLPTRRARTF
DLEVEELHTLVAEGVVVHN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 288 |
| 15N chemical shifts | 137 |
| 1H chemical shifts | 904 |
Additional metadata:
Related Database Links:
| BMRB | 15560 16634 |
| PDB | 2IN0 2IN8 2IN9 2L8L 3IFJ 3IGD |
| EMBL | CFR77613 CMM98736 |
| GB | EPZ65824 KAM73204 |
| REF | WP_049963343 |
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