BMRB Entry 17424
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17424
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Title: Solution NMR Structure of Transmembrane and Cytosolic Regions of Integrin 1 in Detergent Micelles PubMed: 23646163
Deposition date: 2011-01-24 Original release date: 2012-08-14
Authors: Lai, Chaohua; Liu, Xiaoxi; Tian, Changlin
Citation: Lai, Chaohua; Liu, Xiaoxi; Tian, Changlin; Wu, Fangming. "Integrin 1 has a long helix, extending from the transmembrane region to the cytoplasmic tail in detergent micelles." PLoS ONE 8, e62954-e62954 (2013).
Assembly members:
Integrin_Alpha1, polymer, 54 residues, 5272.820 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Integrin_Alpha1: MGLPGRVPLWVILLSAFAGL
LLLMLLILALWKIGFFKRPL
KKKMEKLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 176 |
| 15N chemical shifts | 38 |
| 1H chemical shifts | 202 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Integrin_Alpha1 | 1 |
Entities:
Entity 1, Integrin_Alpha1 54 residues - 5272.820 Da.
| 1 | MET | GLY | LEU | PRO | GLY | ARG | VAL | PRO | LEU | TRP | ||||
| 2 | VAL | ILE | LEU | LEU | SER | ALA | PHE | ALA | GLY | LEU | ||||
| 3 | LEU | LEU | LEU | MET | LEU | LEU | ILE | LEU | ALA | LEU | ||||
| 4 | TRP | LYS | ILE | GLY | PHE | PHE | LYS | ARG | PRO | LEU | ||||
| 5 | LYS | LYS | LYS | MET | GLU | LYS | LEU | GLU | HIS | HIS | ||||
| 6 | HIS | HIS | HIS | HIS |
Samples:
15N-labeled: sodium phosphate 50 mM; DTT 2 mM; Integrin Alpha 1, [U-15N], 1.0 mM; H20 95%; D20 5%
15N-13C-labeled: Integrin Alpha1, [U-13C; U-15N], 1.0 mM; sodium phosphate 50 mM; DTT 2 mM; H20 90%; D20 10%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 15N-labeled | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D HNCO | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D HNCA | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D HNCACB | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D C(CO)NH | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | 15N-13C-labeled | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | 15N-13C-labeled | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAC35370 BAE20498 BAG62150 |
| EMBL | CAA36384 |
| GB | AAI37122 AAI37123 AAI67237 EAW54865 EAW54868 |
| REF | NP_001028400 NP_001296395 NP_112256 NP_852478 XP_001094788 |
| SP | P18614 P56199 Q3V3R4 |
| TPG | DAA17923 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts