BMRB Entry 17474
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17474
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CD147 Ig0 C66M domain. PubMed: 21620857
Deposition date: 2011-02-17 Original release date: 2011-06-01
Authors: Redzic, Jasmina; Armstrong, Geoffrey; Isern, Nancy; Kieft, Jeffrey; Eisenmesser, Elan
Citation: Redzic, Jasmina; Armstrong, Geoffrey; Isern, Nancy; Jones, David; Kieft, Jeffrey; Eisenmesser, Elan Zohar. "The retinal specific CD147 Ig0 domain: from molecular structure to biological activity." J. Mol. Biol. 411, 68-82 (2011).
Assembly members:
CD147_Ig0_C66M, polymer, 137 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CD147_Ig0_C66M: MGSSHHHHHHSSGLVPRGSH
MAGFVQAPLSQQRWVGGSVE
LHCEAVGSPVPEIQWWFEGQ
GPNDIMSQLWDGARLDRVHI
HATYHQHAASTISIDTLVEE
DTGTYECRASNDPDRNHLTR
APRVKWVRAQAVVLVLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 214 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD147 Ig0 C66M_Molecule 1 | 1 |
| 2 | CD147 Ig0 C66M_Molecule 2 | 1 |
Entities:
Entity 1, CD147 Ig0 C66M_Molecule 1 137 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ALA | GLY | PHE | VAL | GLN | ALA | PRO | LEU | SER | ||||
| 4 | GLN | GLN | ARG | TRP | VAL | GLY | GLY | SER | VAL | GLU | ||||
| 5 | LEU | HIS | CYS | GLU | ALA | VAL | GLY | SER | PRO | VAL | ||||
| 6 | PRO | GLU | ILE | GLN | TRP | TRP | PHE | GLU | GLY | GLN | ||||
| 7 | GLY | PRO | ASN | ASP | ILE | MET | SER | GLN | LEU | TRP | ||||
| 8 | ASP | GLY | ALA | ARG | LEU | ASP | ARG | VAL | HIS | ILE | ||||
| 9 | HIS | ALA | THR | TYR | HIS | GLN | HIS | ALA | ALA | SER | ||||
| 10 | THR | ILE | SER | ILE | ASP | THR | LEU | VAL | GLU | GLU | ||||
| 11 | ASP | THR | GLY | THR | TYR | GLU | CYS | ARG | ALA | SER | ||||
| 12 | ASN | ASP | PRO | ASP | ARG | ASN | HIS | LEU | THR | ARG | ||||
| 13 | ALA | PRO | ARG | VAL | LYS | TRP | VAL | ARG | ALA | GLN | ||||
| 14 | ALA | VAL | VAL | LEU | VAL | LEU | GLU |
Samples:
sample_1: Ammonium Chloride, [U-99% 15N], 0.1 w/v; D2O, [U-99% 2H], 100%; glucose, U-13C6, 99%, 0.2 w/v; TRIS 50 mM; sodium chloride 150 mM
2H15N13C_sample: ionic strength: 0.25 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | 2H15N13C_sample |
| 3D 1H-15N NOESY | sample_1 | isotropic | 2H15N13C_sample |
| 3D HNCACB | sample_1 | isotropic | 2H15N13C_sample |
| 3D CBCA(CO)NH | sample_1 | isotropic | 2H15N13C_sample |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment
NMR spectrometers:
- Varian Uniform NMR System 900 MHz
- Varian Uniform NMR System 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts