BMRB Entry 17479

Name: Structure of the human Shwachman-Bodian-Diamond syndrome (SBDS) protein
Published: 2011-05-05

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PDB ID: 2l9n
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17479
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure of the human Shwachman-Bodian-Diamond syndrome (SBDS) protein PubMed: 21536732

Deposition date: 2011-02-21 Original release date: 2011-05-05

Authors: Hilcenko, Christine; Freund, Stefan; Warren, Alan

Citation: Finch, Andrew; Hilcenko, Christine; Basse, Nicolas; Drynan, Lesley; Goyenechea, Beatriz; Menne, Tobias; Gonzalez Fernandez, Africa; Simpson, Paul; Arends, Clive; Donadieu, Mark; Bellanne-Chantelot, Jean; Costanzo, Christine; Boone, Michael; McKenzie, Charles; Freund, Andrew; Warren, Stefan. "Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome." Genes Dev. 25, 917-929 (2011).

Assembly members:
human_SBDS, polymer, 252 residues, 28813.812 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
human_SBDS: GSMSIFTPTNQIRLTNVAVV RMKRAGKRFEIACYKNKVVG WRSGVEKDLDEVLQTHSVFV NVSKGQVAKKEDLISAFGTD DQTEICKQILTKGEVQVSDK ERHTQLEQMFRDIATIVADK CVNPETKRPYTVILIERAMK DIHYSVKTNKSTKQQALEVI KQLKEKMKIERAHMRLRFIL PVNEGKKLKEKLKPLIKVIE SEDYGQQLEIVCLIDPGCFR EIDELIKKETKGKGSLEVLN LKDVEEGDEKFE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	953
15N chemical shifts	248
1H chemical shifts	1642

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	human_SBDS	1

Samples:

sample_1: potassium phosphate 25 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.05%; human_SBDS 0.48 mM; H2O 90%; D2O 10%

sample_2: potassium phosphate 25 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.05%; human_SBDS 0.5 mM; H2O 90%; D2O 10%

sample_3: potassium phosphate 25 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.05%; human_SBDS 0.3 mM; H2O 90%; D2O 10%

sample_4: potassium phosphate 25 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.05%; human_SBDS 0.4 mM; H2O 90%; D2O 10%

sample_5: potassium phosphate 25 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.05%; human_SBDS 0.35 mM; H2O 90%; D2O 10%

sample_6: potassium phosphate 25 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 0.05%; human_SBDS 0.4 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.163 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.163 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_3: ionic strength: 0.163 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_4: ionic strength: 0.163 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_5: ionic strength: 0.163 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_6: ionic strength: 0.163 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_2	isotropic	sample_conditions_2
3D HN(CO)CA	sample_2	isotropic	sample_conditions_2
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_2
3D HNCACB	sample_2	isotropic	sample_conditions_2
3D HNCACO	sample_2	isotropic	sample_conditions_2
3D HNCA	sample_5	isotropic	sample_conditions_5
3D HNCO	sample_5	isotropic	sample_conditions_5
3D HN(CO)CA	sample_5	isotropic	sample_conditions_5
3D HBHA(CO)NH	sample_5	isotropic	sample_conditions_5
3D HNCACB	sample_5	isotropic	sample_conditions_5
3D HNCACO	sample_5	isotropic	sample_conditions_5
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_3
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_6	isotropic	sample_conditions_6
2D 1H-13C HSQC	sample_6	isotropic	sample_conditions_6
3D HCCH-TOCSY	sample_6	isotropic	sample_conditions_6
3D HCCH-COSY	sample_6	isotropic	sample_conditions_6
3D HN(CAN)NH	sample_2	isotropic	sample_conditions_2
3D (H)CC(CO)NH	sample_2	isotropic	sample_conditions_2
3D H(CCCO)NH	sample_2	isotropic	sample_conditions_2

BMRB	16119
PDB	2KDO 2L9N 5AN9 5ANB 5ANC
DBJ	BAA91905 BAB23803 BAB29487 BAB31612 BAE40670
EMBL	CAH91068
GB	AAH03849 AAH65700 AAH86335 AAI04586 AAN77490
REF	NP_001008290 NP_001029611 NP_001125618 NP_001231322 NP_057122
SP	P70122 Q3SWZ6 Q5RAZ2 Q5RK30 Q9Y3A5
TPG	DAA15388

Biological Magnetic Resonance Data Bank

BMRB Entry 17479

Title: Structure of the human Shwachman-Bodian-Diamond syndrome (SBDS) protein PubMed: 21536732

Additional metadata:

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