BMRB Entry 17490

Assembly:

Entities:

Entity 1, L4W 117 residues - 12568.729 Da.

Samples:

13C15N_H2O: L4W, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6 18 mM; potassium chloride 45 mM; DTT 0.9 mM; H2O 9%; D2O 10%

13C15N_D2O: L4W, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6, [U-99% 2H], 18 mM; potassium chloride, [U-99% 2H], 45 mM; D2O 100%

Reference: L4W, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6 18 mM; potassium chloride 45 mM; DSS 1 uM; H2O 90%; D2O 10%

Unlabeled_D2O: L4W 600 uM; potassium phosphate pH 6, [U-99% 2H], 18 mM; potassium chloride, [U-99% 2H], 45 mM; D2O 100%

Aligned: L4W, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6 18 mM; potassium chloride 45 mM; DTT 0.9 mM; DMPC/DHPC q=3 6.5%; CTAB 0.67 mg/mL; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

RDCs: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

Software:

VNMRJ, Varian - collection

xwinnmr, Bruker Biospin - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

HADDOCK v2.0, Alexandre Bonvin - data analysis, refinement

UNIO v08, Torsten Herrmann - structure solution

nmr_structure_tools, Lawrence J Clos - data analysis

NMR spectrometers:

• Varian Uniform NMR System 600 MHz
• Bruker DMX 750 MHz
• Varian Uniform NMR System 900 MHz